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| The effect of Qingxin Kaiqiao Formula on neuroinflammation in APP/PS1 double transgenic mice based on NLRP3 inflammasome |
| WANG Liuying1, 2, LAI Xiaoxiao2, LIU Shuo1, 2, XU Luting2, SHEN Yan2, HU Haiyan1, 2. |
| 1.Department of Traditional Chinese Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.The Second Clinical Medical College, Wenzhou Medical University,Wenzhou 325035, China |
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| Cite this article: |
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WANG Liuying,LAI Xiaoxiao,LIU Shuo, et al. The effect of Qingxin Kaiqiao Formula on neuroinflammation in APP/PS1 double transgenic mice based on NLRP3 inflammasome[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(6): 431-439.
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Abstract Objective: To investigate the effect of the Qingxin Kaiqiao Formula on neuroinflammation and cognitive performance in mice with Alzheimer’s disease (AD) model and to explore its association with NLRP3 inflammasome-related pathways. Methods: APP/PS1 double transgenic mice were randomly divided as model group, Aricept treatment group (1.67 mg·kg-1·d-1), and Qingxin Kaiqiao Formula treatment group (low dose group: 4.75 mg·kg-1·d-1; middle dose group: 9.5 mg·kg-1·d-1; high dose group: 19 mg·kg-1·d-1), with ten mice in each group. SPF-grade male C57BL/6J mice served as the control group. The Morris water maze experiment was performed to test the ability of directional navigation and spatial perception; HE and Nissl staining were performed to determine changes in the number of cells and Nissl bodies in the hippocampal region; Western blot and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) were used to determine the expression of NLRP3, Caspase-1, Aβ, IL-1β, and IL-6. Results: Compared with the control group, mice in the model group showed significantly increased mean escape latency (P<0.01), significantly decreased number of passage through the plateau shorter residence time in the target quadrant where the plateau was located (P<0.01),decreased number of neurons in the hippocampal region, irregularity, decreased number of Nissl bodies , lighter staining color, and obviously increased expression of NLRP3, caspase-1, Aβ, IL-1β and IL-6 (P<0.01). Compared with the model group, both the Aricept treatment group and the Qingxin Kaiqiao Fang-treated group showed visibly shorter escape latency, significantly greater number of crossing platforms longer residence time in the quadrant where the plateau was located (P<0.05 or P<0.01); and the neuronal cells in the treated group were more closely arranged and more intact morphologically, apparently higher number of Nissl bodies deeper color,and significantly reduced NLRP3, Caspase-1, Aβ, IL-1β and IL-6 expression (P<0.05 or P<0.01). Conclusion:Qingxin Kaiqiao Formula may improve Aβ pathological changes and nimpairment in learning and memory in APP/PS1 mice by reducing neuroinflammation through inhibition of the NLRP3 inflammatory pathway.
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Received: 26 February 2023
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