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| The role of miRNA-1 in cardiomyocyte pyroptosis and injury after cardiac arrest cardiopulmonary resuscitation in rats |
| YIN Jiana1, LI Bingcan1, ZHOU Peisen2, LEI Yuanli1, WANG Dongsheng1, XU Huaqing1, SONG Wenxing1, HE Aiwen1, LI Zhangping3 |
| 1.Department of Emergency, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Department of Emergency, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China; 3.Department of Emergency, the Quzhou Hospital Affiliated to Wenzhou Medical University, Quzhou People’s Hospital, Quzhou 324000, China |
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| Cite this article: |
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YIN Jiana,LI Bingcan,ZHOU Peisen, et al. The role of miRNA-1 in cardiomyocyte pyroptosis and injury after cardiac arrest cardiopulmonary resuscitation in rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(1): 29-35，41.
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Abstract Objective: To investigate the role of miR-1 in myocardial pyroptosis and injury after cardiac arrest/cardiopulmonary resuscitation (CA/CPR) in rats. Methods: Cardiac arrest/cardiopulmonary resuscitation model was establishment and Spragu-Dawley (SD) rats were divided as cardiac arrest resuscitation (CA) group and sham operation control (Sham) group according to random number table method. The former was subdivided into 5 groups: CA group, CA+antago miR-1 group, CA+antago miR NC group, CA+ago miR-1 group, CA+ago miR NC group (n=6). All cardiac apical myocardial tissue and blood samples of all 6 groups were tested 6 hours after ROSC. The mRNA level of miRNA-1 in each group was detected using Real-time PCR. The expression of pyroptosis-related proteins including NLRP3, Caspase-1, IL-1β and IL-18 in each group was detected by Western blot. The pyroptosis rate of cardiomyocytes was detected by Tunel stain assay.The concentration of CK-MB and cTnI in serum was detected by ELISA. Results: There were no significant difference in body weight and basic MAP among the groups (P>0.05). Compared with Sham group, CA group had an increased expression of miR-1, NLRP3, Caspase-1, IL-1β, IL-18, pyroptosis rate, serum CK-MB and cTnI concentration (P<0.05). Compared with CA group, CA+antago miR-1 group had decreased expression of miR-1, NLRP3, Caspase-1, IL-1β, IL-18, pyroptosis rate, serum CK-MB and cTnI concentration (P<0.05); the expression of miR-1 and the expression of myocardial pyroptosis related proteins NLRP3, Caspase-1, IL-1β, IL-18, pyroptosis rate, serum CK-MB and cTnI concentration increased in CA+ago miR-1 group (P<0.05); there was no significant change in the expression of miR-1, NLRP3, Caspase-1, IL-1β, IL-18, pyroptosis rate, serum CK-MB and cTnI concentration in CA+antago miR NC group and CA+ago miR NC group (P>0.05). Conclusion: There was obvious myocardial pyroptosis and injury in CA/CPR rats after ROSC. Intravenous administration of ago miR-1 and antago miR-1 could regulate the expression of miR-1 in rat cardiomyocytes after CA/CPR, and miR-1 promoted myocardial pyroptosis and injury in rats after CA/CPR. The mechanism of miR-1 regulating myocardial injury after CA/CPR might be related to its promotion of myocardial pyroptosis.
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