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| Ectopic study of calcium phosphate cement seeded with antagomir-30d transfected BMSCs |
| CHEN Wei1, WANG Yan1, JIN Qiong2, HUANG Hui3 |
| 1.Department of Pediatric Dentistry, School & Hospital of Stomatology Wenzhou Medical University, Wenzhou 325000, China; 2.Department of Implant Prosthodontics, School & Hospital of Stomatology Wenzhou Medical University, Wenzhou 325000, China; 3.Department of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China |
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| Cite this article: |
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CHEN Wei,WANG Yan,JIN Qiong, et al. Ectopic study of calcium phosphate cement seeded with antagomir-30d transfected BMSCs[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(1): 1-6.
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Abstract Objective: To constructed a compound of antagomir-30d/bone marrow mesenchymal stem cells (BMSCs)/calcium phosphate cement (CPC) and implant s.c. in BALB/c-nu mice to demonstrate that antagomir-30d could promote ectopic osteogenesis. Methods: The BMSCs transfected with the optimum concentration of antagomir-30d/NC/blank was loaded on calcium phosphate cement scaffolds and s.c. was implanted into BALB/c-nu mice. Implants were removed after 2, 4, 8 week (wk) respectively. 2-wk implants were subjected to RNA extraction, and then gene expression of osteoblast marker genes alkaline phosphatase (ALP), osteocalcin (OC) and Runt-related transcription factor 2 (RUNX2) was analyzed. 4-wk and 8-wk implants were subjected to
histological staining and histomorphometric analysis was made to observe new bone formation. Results: Gene expression of osteoblast marker genes was analyzed after 2 wk implantation. RT-PCR analysis revealed up-regulation of ALP, OC and RUNX2 in the antagomir-30d-treated implants compared with implants transfected with miR negative control and the un-transfected group. The histological staining revealed that the antagomir-30d-treated implants had a small amount of new bone formation at 4 wk. Implants transfected with miR negative control and the un-transfected group showed new bone formation barely. Histomorphometric analysis showed that the percentage of new bone area of antagomir-30d-treated implants was 1.28%±0.19%. At 8 wk, new bone formation was clearly visible in all groups, while the new bone formation of antagomir-30d-treated group was more significant than NC and un-transfected groups (P<0.05). New bone area percentage was 17.79%±1.15%, 1.82%±0.53%, 2.33%±0.52% respectively. Conclusion: Antagomir-30d accelerates ectopic bone formation in vivo, and a compound of antagomir-30d/BMSCs/CPC is promising as tissue engineering complexes in repairing jaw bone defects.
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