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| Distribution characteristics of Pseudomonas aeruginosa T3SS and T6SS and their correlation with drug resistance |
| ZHOU Beibei1, WANG Lingbo1, ZHANG Xiucai2, CHEN Lijiang1, WANG Zhongyong1,ZHOU Tieli1. |
| 1.Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015, China; 2.Department of Clinical Laboratory, the Children’s Hospital of Zhejiang University School of Medicine, Hangzhou 310052, China |
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| Cite this article: |
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ZHOU Beibei,WANG Lingbo,ZHANG Xiucai, et al. Distribution characteristics of Pseudomonas aeruginosa T3SS and T6SS and their correlation with drug resistance[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(12): 981-986.
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Abstract Objective: To understand the Type III secretion system (T3SS) and Type VI secretion system(T6SS) gene distribution characteristics of Pseudomonas aeruginosa (P. aeruginosa) from sputamentum and blood and their correlation with drug resistance. Methods: A total of 92 strains of P. aeruginosa (61 strains isolated from sputamentum, and 31 strains isolated from blood) in the First Affiliated Hospital of Wenzhou Medical University were collected from 2016 to 2018. The PCR method was used to detect the T3SS effector protein encoding genes exoS, exoT, exoU, exoY and pscC and the T6SS effector protein encoding genes tssB1, tse1, tse2,tse3, tssB2, pldA, tssB3, and pldB. The agar dilution method was used to detect the sensitivity of the strains to commonly used clinical antibacterial drugs. The correlation between different infections and the carrying status of genes encoded by the secretory system was investigated, and further analysis of their relationship with drug
resistance was made. Results: The detection rate of T3SS protein encoding gene exoT and T6SS protein genes tssB1, tse2, tssB2, and pldB in sputamentum and blood was as high as > 90%. In addition, the carrying rate of T3SS protein gene exoU in strain from sputum was significantly higher than from blood (P<0.05), and the
drug resistance rate of strains to imipenem derived from sputum was significantly higher than those from blood (P<0.05). Spearman correlation analysis showed exoU was positively correlated to pldA (r=0.474, P<0.01).There was no significant difference among strains with different T3SS and T6SS gene profiles against commonly
antibiotics (P>0.05). Conclusion: The pldA gene often coexists with the virulence gene exoU in P. aeruginosa. The carrying rate of T3SS effector protein encoding gene exoU and the resistance against imipenem in P. aeruginosa from sputum is significantly higher than that of strains from blood.
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Received: 26 May 2022
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. [J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(7): 595-597. |
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