白细胞介素17对大鼠肝星状细胞胶原代谢的影响

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Abstract Objective: To investigate the effect of Interleukin17 (IL-17) on proliferation and expression of MMP-9, TIMP-1 in rat hepatic stellate cells in vitro. Methods: The HSCs were isolated by method of Optiprep. Immunocytochemical method was used to detect the α-SMA expression of HSCs. The HSCs was treated with different concentrations of IL-17 and the proliferation of HSCs was measured by MTT. The MMP-9 and TIMP-1 mRNA levels were detected by reverse-transcription polymerase chain reaction (RT-PCR). While the MMP-9 and TIMP-1 protein were measured by ELISA. Results: HSCs were isolated and cultured successfully. Stimulated with IL-17 could significantly increase proliferation and α-SMA expression in HSCs. IL-17 could promote the MMP-9 and TIMP-1 mRNA expression and increased protein secresion in HSCs by dose depedent manner. With increased of IL-17 concentration, the value of TIMP-1/MMP-9 was markedly upregulated. Conclusion: IL-17 can promote the proliferation of HSCs and also increases α-SMA, MMP-9 and TIMP-1 expression, then upregulate the value of TIMP-1/MMP-9 in HSCs, which may play an important role in live fibrosis.

Key words： interleukin17 hepatic stellate cells α-SMA MMP-9 TIMP-1 rats

Received: 15 May 2014

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	YU Panpan1
	ZHANG Qiyu2
	JIN Huicheng1.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I12/868

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