|
|
|
| Curcumin regulates LncRNA MEG3/miR-21/PTEN pathway to promote the apoptosis of HPV-positive cervical cancer cells |
| HUANG Ying, SHI Yaoqi, HU Jiaming, NI Xinyu, ZHU Hua |
| Department of Gynecology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China |
|
| Cite this article: |
|
HUANG Ying,SHI Yaoqi,HU Jiaming, et al. Curcumin regulates LncRNA MEG3/miR-21/PTEN pathway to promote the apoptosis of HPV-positive cervical cancer cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(2): 115-120.
|
|
|
Abstract Objective: To explore the mechanism of curcumin in killing HPV-positive cervical cancer cells. Methods: First, the effect of curcumin on the viability and apoptosis of HPV-positive cervical cancer cells were analyzed by cell viability detection experiment and apoptosis detection experiment; then, real-time quantitative PCR experiment and Western blot were used to analyze the changes in key signal pathways of curcumin in the process of promoting the apoptosis of HPV-positive cervical cancer cells. Results: Curcumin significantly promoted the apoptosis of HPV-positive cervical cancer cells, increased the expression levels of LncRNA MEG3, PTEN and activated caspase3, and down-regulated the expression of miR-21; and knocking down the expression
of LncRNA MEG3 by siRNA ccould reverse the regulation of curcumin on miR-21, PTEN and caspase3. Conclusion: Curcumin may decrease the expression of miR-21 by up-regulating LncRNA MEG3, thereby up-regulating the expression of PTEN, and ultimately promote the apoptosis of HPV-positive cervical cancer cells.
|
|
|
| |
|
|
|
|
|
|
