富氢水抗1型糖尿病小鼠肾脏氧化应激的作用

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (16944 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To study the protective effect of hydrogen on renal injury induced by type 1 dibetes in mice and explore the possible mechanism. Methods: Sixty healthy male ICR mice were randomly divided into control group (A), diabetic group (B) and diabetes hydrogen water intervention group (C). Diabetes were induced by intraperitoneal injection of streptozotocin 150 mg/kg. Group C were injected saturated hydrogen-rich water 5 mL/kg and group B were injected with equal normal saline every day for 8 weeks in mice models of type 1 dibetes. The blood was collected from the tail vein and blood glucose was measured. Renal injury were identified by hematoxylin and eosin (HE) staining. Expression of Nox4 and HO-1 were identified by immunohistochemical staining. Results: Expression of Nox4 in Group C was significantly lower than that in group B, expression of HO-1 in Group C was significantly higher than that in group B. Blood glucose in Group B and C were higher than that in Group A, but there was no difference between the two groups. Kidney HE staining did not show significant lesions. Conclusion: Hydrogen-rich water can reduce oxidative stress in renal tissue of diabetic mice.

Key words： hydrogen-rich water dabetic nephropathy oxidative stress mice

Received: 20 March 2014

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHAO Chenchen1
	WANG Qingqing1
	QIU Pengcheng1
	ZHU Penglei1
	SU Zhongqian1
	MAO Yuyan2
	SONG Zhangjuan3
	JIN Keke3.

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I11/813

[1]Bedard K, Krause KH. The NOX family of ROS-generat-ing NADPH oxidases: physiology and pathophysiology[J].Physiol Rev, 2007, 87(1): 245-313.
[2]Ohsawa I, Ishikawa M, Takahashi K, et al. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals[J]. Nat Med, 2007, 13(6): 688-694.
[3]吴垣辕, 刘东方. Nox和氧化应激与糖尿病[J]. 中国生物化学与分子, 2013, 29(1): 7-12.
[4]Yu YS, Zheng H. Chronic hydrogen-rich saline treatment reduces oxidative stress and attenuates left ventricular hyper-trophy in spontaneous hypertensive rats[J]. Mol Cell Bio-chem, 2012, 365(1-2): 233-242.
[5]Zhai X, Chen X, Shi J, et al. Lactulose ameliorates cerebral ischemia-reperfusion injury in rats by inducing hydrogen by activating Nrf2 expression[J]. Free Radic Biol Med, 2013,65: 731-741.
[6]Kawamura T, Wakabayashi N, Shigemura N, et al. Hydrogen gas reduces hyperoxic lung injury via the Nrf2 pathway in vivo[J]. Am J Physiol Lung Cell Mol Physiol, 2013, 304(10): L646-656.
[7]Chihiro S, Hironori K, Satoshi H, et al. Hydrogen-rich saline solution attenuates renal ischemia-reperfusion in jury[J]. J Anesth, 2010, 24(4): 569-574.
[8]Wang F, Yu G, Liu SY, et al. Hydrogen-rich saline protects against renal ischemia/reperfusion injury in rats[J]. J Surg Res, 2011, 167(2): e339-344.
[9]Kamimura N, Nishimaki K, Ohsawa I, et al. Molecular hy-drogen improves obesity and diabetes by inducing hepatic FGF21 and stimulating energy metabolism in db/db mice[J]. Obesity, 2011, 19(7): 1396-1403.
[10]Kajiyama S, Hasegawa G, Asano M, et al. Supplementation of hydrogen-rich water improves lipid and glucose me-tabolism in patients with type 2 diabetes or impaired glucose tolerance[J]. Nutr Res, 2008, 28(7): 137-143.
[11]Xiao X, Cai J, Xu J, et al. Protective effects of hydrogen sa-line on diabetic retinopathy in a streptozotocin-induced dia-betic rat model[J]. J Ocul Pharmacol Ther, 2012, 28(1): 76-82.