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| Kaempferide inhibits the proliferation and migration of lung cancer cell A549 by down-regulating circ-RPL15 |
| ZHENG Chengchao1, MAO Jiabin1, HE Daqiang2 |
| 1.Department of Tuberculosis, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China; 2.Department of Laboratory, Hangzhou First People’s Hospital Affiliated to Medical College of Zhejiang University, Hangzhou 310006, China |
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| Cite this article: |
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ZHENG Chengchao,MAO Jiabin,HE Daqiang. Kaempferide inhibits the proliferation and migration of lung cancer cell A549 by down-regulating circ-RPL15[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(12): 960-967.
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Abstract Objective: To explore whether kaempferide can affect the biological behavior of lung cancer cell A549 through circ-RPL15. Methods: Kaempferide of 25, 50, and 75 μg/mL was used to treat lung cancer cells A549. The dual luciferase report experiment was used to analyze the targeted regulation of miR-489-3p by circ-RPL15. circ-RPL15 was silenced or overexpressed in cell A549, and treated with 75 μg/mL kaempferol. Cell counting kit-8 (CCK-8) was applied to measure changes in the viability of lung cancer cells A549 in each group;plate cloning was performed to detect cell clone formation; flow cytometry was used to monitor cell apoptosis;Western blot was employed to detect the expression of Cleaved-caspase3 protein; Transwell method was used to detect cell migration, and real-time quantitative polymerase chain reaction (RT-qPCR) to determine the expression of circ-RPL15 and miR-489-3p. The dual luciferase report experiment analyzed the targeted regulation of miR-489-3p by circ-RPL15. Results: Compared with the control group, after different concentrations (25, 50 and 75 μg/mL) of kaempferide acted on lung cancer cell A549, cell viability, clone formation number, migration number and circ-RPL15 expression decreased, while apoptosis rate, Cleaved-caspase3 protein and miR-489-3p expression increased (P<0.05), and all were concentration-dependent. Silencing circ-RPL15 enhanced the expression of miR-489-3p, apoptosis rate and Cleaved-caspase3 protein expression of lung cancer cell A549, and weakened cell viability, the number of clone formation, and the number of migration (P<0.05). circ-RPL15 targets miR-489-3p. Overexpression of circ-RPL15 reversed the proliferation, apoptosis and migration of lung cancer cells that were affected by kaempferide (P<0.05). Conclusion: Kaempferide inhibits the proliferation and migration of lung cancer cells and induces their apoptosis by down-regulating circ-RPL15 and targeting miR-489-3p.
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Received: 16 July 2021
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