同一胎鼠肠和脑神经干细胞神经递质的表达

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Abstract Objective: To observe the expression of neurotransmitters in enterogenous neural stem cells (ENSCs) and central nervous system neural stem cells (CNS-NSCs) from the same fetal rats, and to explore a suitable stem cell source for neural stem cells transplantation. Methods: Rat ENSCs and CNS-NSCs were extracted from the same 20-day SD rat embryos followed by repeated subculture using improved neural stem cell medium. Two different kinds of nerve bulbs originated from 6-10 days culturing were chosen. The expressions of four transmitters on ENSCs and CNS-NSCs were analyzed by agarose pre-embedded immunocytochemistry assay and flow cytometry. Results: After analyzing data of imunocytochemistry assay, we found the expression of nNOS and ChAT on ENSCs; nNOS, ChAT and DβH on CNS-NSCs. Significant differences of nNOS and ChAT expression were observed between ENSCs and CNS-NSCs respectively (P<0.05). Flow cytometry analysis indicated GABA(+), nNOS(+), ChAT(+), DβH(+) cells averagely accounted for 1.59%, 14.97%, 5.43%, 1.36% in ENSCs respectively,within which nNOS and ChAT expression were significantly different from isotype groups (P<0.05). In CNS-NSCs, the pecentages were 1.68%, 6.14%, 25.42%, 9.30% on average, comparing with isotype groups, the differences of nNOS, ChAT, and DβH expression were all significant (P<0.05). Conclusion: ENSCs express neurotransmitters including NO and Ache, with NO of dominance. While CNS-NSCs express neurotransmitters such as NO, Ache and NE, especially Ache. There is almost no GABA expression in either ENSCs or CNS-NSCs.

Key words： NSCs neurotransmitter expression fetal rats

Received: 31 August 2014

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	XIA Liguang
	LI Zhongrong
	LIU Senqing
	WANG Yongbiao
	ZHU Libin
	ZHAO Yiming.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I11/792

[1]朱利斌, 刘征吉, 王爱和, 等. 大鼠肠神经干细胞体外培养和鉴定[J]. 中华实验外科杂志, 2007, 24(1): 122-122.
[2]夏立广, 黄婷, 黄晓忠, 等. 肠和脑来源神经干细胞的nNOS表达研究[J]. 医学研究杂志, 2012, 41(9): 58-61.
[3]Pontes A, Zhang Y, Hu W. Novel functions of GABA signaling in adult neurogenesis[J]. Front Biol, 2013, 8(5): 496-507.
[4]Weigert N, Schepp W, Haller A, et al. Regulation of gastrin, somatostatin and bombesin release from the isolated rat stomach by exogenous and endogenous gamma-aminobut-vric acid[J]. Digestion, 1998, 59(1): 16-25.
[5]Abbas WR, Maiti RN, Goel RK, et al. Effect of GABA and baclofen on gastric mucosal protective factors[J]. Indian J Exp Biol, 1998, 36(2): 182-186.
[6]Marcoli M, Scarrone S, Maura G, et al. A subtype of gam-ma-aminobutvric acid(B) receptor regulates cholinergic response in the guinea pig ileum[J]. Pharmacol Exp Ther, 2000, 293(1): 42-47.
[7]Miyoshi G, Bessho Y, Yamada S, et al. Identification of a novel basic helix-loop-helix gene, Heslike, and its role in GABAergic neurogenesis[J]. Neurosci, 2004, 24(14): 3672-3682.
[8]陈锦华, 杨辉, 尹昌林, 等. BMP2在SVZa神经干细胞向GABA能神经元分化中的调控作用[J]. 第三军医大学学报, 2007, 29(8): 699-701.
[9]Estrada C, Murillo-Carretero M. Nitric oxide and adult neu-rogenesis in health and disease[J]. Neuroscientist, 2005,11(4): 294-307.
[10]萨喆燕, 郭试瑜, 单立冬, 等. 一氧化氮促进体外培养大鼠脑室下区神经干细胞的分化[J]. 中国应用生理学杂志,2010, 26(3): 359-364.
[11]Gibbs SM. Regulation of neuronal proliferation and differentiation by nitric oxide[J]. Mol Neurobiol, 2003, 27(2): 107-120.
[12]Luo CX, Jin X, Cao CC, et al. Bidirectional regulation of neurogenesis by neuronal nitric oxide synthase derived from neurons and neural stem cells[J]. Stem Cells, 2010, 28(11): 2041-2052.
[13]Thatte HS, He XD, Goyal RK. Imaging of nitric oxide in nitrergic neuromuscular neurotransmission in the gut[J]. PloS One, 2009, 4(4): e4990.
[14]Belknap WM. Hirschsprung’s disease[J]. Curr Treat Options Gastroenterol, 2003, 6(3): 247-256.
[15]Camilleri M. Diagnosis and treatment of enteric neuromus-cular diseases[J]. Clin Auton Res, 2003, 13(1): 10-15.
[16]Chelimsky G, Chelimsky TC. Evaluation and treatment of autonomic disorders of the gastrointestinal tract[J]. Semin Neurol, 2003, 23(4): 453-458.
[17]Winkler J, Suhr ST, Gage FH, et al. Essential role of neocor-tical acetylcholine in spatial memory[J]. Nature, 1995, 375(6531): 484-487.
[18]Garibotto V, Tettamanti M, Marcone A, et al. Cholinergic activity correlates with reserve proxies in Alzheimer’s disease[J].Neurobiol Aging, 2013, 34(11): 2694.e13-18.
[19]Zhou C, Wen ZX, Shi DM, et al. Muscarinic acetylcholine receptors involved in the regulation of neural stem cell pro-liferation and differentiation in vitro[J]. Cell Biol Int, 2004,28(1): 63-67.
[20]Zhu P, Li H, Jin G, et al. LIM-homeobox gene Lhx8 promote the differentiation of hip campal newborn neurons into cholinergic neurons in vitro[J]. In Vitro Cell Dev Biol Anim, 2013, 49(2): 103-107.
[21]Thomas SA, Palmiter RD. Thermoregulatory and metabol-ic phenotypes of mice lacking noradrenaline and adrenaline[J]. Nature, 1997, 387(6628): 94-97.
[22]蔡一亭, 秦新裕. 根治性胃大部分切除术后胃瘫15例临床分析[J]. 中国实用外科杂志, 1999, 19(6): 338-340.
[23]Mong J, Panman L, Alekseenko Z, et al. Transcription factor-induced lineage programming of noradrenaline and motor neurons from embryonic stem cells[J]. Stem Cells, 2014, 32(3): 609-622.

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