人支气管上皮细胞中苯并芘诱导TNF-α表达的分子机制

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Abstract Objective: To study B[a]P on TNF-α mRNA expression regulation mechanism in Beas-2B cells through constructing TNF-α promoter luciferase reporter gene vector. Methods: The expression of TNF-α mRNA was detected by Real time-PCR with in 24 hours in Beas-2B cells exposed to B[a]P. We constructed TNF-α promoter luciferase reporter gene vector by molecular cloning technique and detected the promoter activity. Also we detected the change trend of TNF-α promoter activity in Beas-2B cells and 293T cells exposed to B[a]P. Results: Upon B[a]P exposure, the expression of TNF-α mRNA increased gradually with in 24 hours in Beas-2B cells and B[a]P can induce the TNF-α promoter activity. The TNF-α promoter activity also enhanced in 293Tcells exposed to B[a]P environment. Conclusion: We successfully construct the TNF-α promoter luciferase reporter gene plasmids and explained that B[a]P can induce the expression of TNF-α mRNA at transcriptional level without cell specificity.

Key words： TNF-α the promoter luciferase reporter gene Beas-2Bcells B[a]P lung tumor

Received: 15 July 2014

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	DAI Jia
	LV Jianyi
	ZHANG Min
	CAI Qinzhen
	GAO Jimin.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I11/781

[1]Wu Y, Zhou BP. TNF-alpha/NF-kappaB/Snail pathway in cancer cell migration and invasion[J]. Br J Cancer, 2010, 102(4): 639-644.
[2]Mathew SJ, Haubert D, Krönke M, et al. Looking beyond death: a morphogenetic role for the TNF signalling pathway[J]. J Cell Sci, 2009, 122(12): 1939-1946.
[3]Kruglov AA, Kuchmiy A, Grivennikov SI, et al. Physiological functions of tumor necrosis factor and the consequences of its pathologic overexpression or blockade: mouse models[J]. Cytokine Growth Factor Rev, 2008, 19(3-4): 231-244.
[4]Idriss HT, Naismith JH. TNF alpha and the TNF receptor superfamily: structure-function relationship(S)[J]. Microsc Res Tech, 2000, 50(3): 184-195.
[5]Pfeffer K. Biological functions of tumor necrosis factor cytokines and their receptors[J]. Cytokine Growth Factor Rev, 2003, 14(3-4): 185-191.
[6]Greenberg AK, Tsay JC, Tchou-Wong KM, et al. Chemoprevention of lung cancer: prospects and disappointments in human clinical trials[J]. Cancers(Basel), 2013, 5(1): 131-148.
[7]Hecht SS. Tobacco smoke carcinogens and lung cancer[J]. J Natl Cancer Inst, 1999, 91(14): 1194-1210.
[8]Cosman M, de los Santos C, Fiala R, et al. Solution conformation of the major adduct between the carcinogen(+)-anti-benzo[a]pyrene diol epoxide and DNA[J]. Proc Natl Acad Sci USA, 1992, 89(5): 1914-1918.
[9]Jiang H, Gelhaus SL, Mangal D, et al. Metabolism of benzo[a]pyrene in human bronchoalveolar H358 cells using liquid chromatography-mass spectrometry[J]. Chem Res Toxicol, 2007, 20(9): 1331-1341.
[10]Newell CL, Deisseroth AB, Lopez-Berestein G. Interaction of nuclear proteins with an AP-1/CRE-like promoter sequence in the human TNF-alpha gene[J]. J Leukoc Biol, 1994, 56(1): 27-35.
[11]Lara-Sampablo A, Flores-Alonso JC, De Jesus-Ortega N, et al. Transfection of influenza A virus nuclear export protein induces the expression of tumor necrosis factor alpha[J]. Virus Res, 2014, 185: 1-9.
[12]Coussens LM, Werb Z. Inflammation and cancer[J]. Nature, 2002, 420(6917): 860-867.
[13]徐颖颖, 王吉耀. 乙型肝炎、肝硬化与肝癌[J]. 临床内科杂志, 2009, 26(11): 728-730.
[14]江宏恩, 李鸿娟. 胃溃疡与胃癌[J]. 中国实用外科杂志, 1998, 18(8): 500-501.
[15]陈赛斐. HPV在宫颈炎、宫颈癌前病变、宫颈癌中的检测及意义分析[J]. 中国妇幼保健, 2011, 26(15): 2392-2393.
[16]Balkwill F. Tumour necrosis factor and cancer[J]. Nat Rev Cancer, 2009, 9(5): 361-371.
[17]Karin M, Greten FR. NF-kappaB: linking inflammation and immunity to cancer development and progression[J]. Nat Rev Immunol, 2005, 5(10): 749-759.
[18]Hsu TC, Nair R, Tulsian P, et al. Transformation nonresponsive cells owe their resistance to lack of p65/nuclear factor-kappaB activation[J]. Cancer Res, 2001, 61(10): 4160-4168.
[19]Shishodia S, Aggarwal BB. Nuclear factor-κB activation mediates cellular transformation, proliferation, invasion angiogenesis and metastasis of cancer[J]. Cancer Treat Res, 2004, 119: 139-173.
[20]Johnston DA, Dong B, Hughes CC. TNF induction of jagged-1 in endothelial cells is NF kappaB-dependent[J]. Gene, 2009, 435(1-2): 36-44.
[21]Leibovich SJ, Polverini PJ, Shepard HM, et al. Macrophage-induced angiogenesis is mediated by tumour necrosis factor-alpha[J]. Nature, 1987, 329(6140): 630-632.
[22]Hagemann T, Wilson J, Kulbe H, et al. Macrophages induce invasiveness of epithelial cancer cells via NF-kappaB and JNK[J]. J Immunol, 2005, 175(2): 1197-1205.
[23]Orosz P, Echtenacher B, Falk W, et al. Enhancement of experimental metastasis by tumor necrosis factor[J]. J Exp Med, 1993, 177(5): 1391-1398.
[24]Kulbe H, Hagemann T, Szlosarek PW, et al. The inflammatory cytokine tumor necrosis factor-alpha regulates chemo-kine receptor expression on ovarian cancer cells[J]. Cancer Res, 2005, 65(22): 10355-10362.
[25]Liang M, Zhang P, Fu J. Up-regulation of LOX-1 expression by TNF-α promotes trans-endothelial migration of MDA-MB-231 breast cancer cells[J]. Cancer Lett, 2007, 258(1): 31-37.
[26]Tran TA, Kallakury BV, Ambros RA, et al. Prognostic significance of tumor necrosis factor and their receptors in non-small cell lung carcinoma[J]. Cancer, 1998, 83(2): 276-282.
[27]贾彦民, 陈明伟, 马建光. 肺癌TNF-α的表达及其意义[J].西安医科大学学报, 2000, 21(5): 425-427.
[28]Gaensler EA, Carrington CB. Open biopsy for chronic diffuse infiltrative lung disease: clinical, roentgenographic, and physiological correlations in 502 patients[J]. Ann Thorac Surg,1980, 30(5): 411-426.
[29]Xu H, Verbeken E, Vanhooren HM, et al. Pulmonary toxicity of polyvinyl chloride particles after a single intratracheal instillation in rats. Time course and comparison with silica[J]. Toxicol Appl Pharmacol, 2004, 194(2): 111-121.
[30]Nelson SM, Hajivassiliou CA, Haddock G, et al. Rescue of the hypoplastic lung by prenatal cyclical strain[J]. Am J Respir Crit Care Med, 2005, 171(12): 1395-1402.
[31]Ding J, Huang Y, Ning B, et al. TNF-alpha induction by nickel compounds is specific through ERKs/AP-1-dependent pathway in human bronchial epithelial cells[J]. Curr Cancer Drug Targets, 2009, 9(1): 81-90.
[32]Ouyang W, Hu Y, Li J, et al. Direct evidence for the critical role of NFAT3 in benzo[a]pyrene diol-epoxide-induced cell transformation through mediation of inflammatory cytokine TNF induction in mouse epidermal Cl41 cells[J]. Carcino-genesis, 2007, 28(10): 2218-2226.
[33]丘创华, 候敢, 黄迪南. TNF-α信号传导通路的分子机制[J]. 中国生物化学与分子生物学报, 2007, 23(6): 430-435.