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| The effect of lncRNA TTN-AS1 targeted inhibition of miR-1271 expression on the proliferation, invasion and apoptosis of lung cancer cells and its mechanism |
| CHEN Zhe1, WANG Qinning1, HUANG Lujie1, SHI Xinbao1, HU Jing2 |
| 1.Department of Cardiothoracic Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo 315040; 2.Department of Radiotherapy, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China |
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| Cite this article: |
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CHEN Zhe,WANG Qinning,HUANG Lujie, et al. The effect of lncRNA TTN-AS1 targeted inhibition of miR-1271 expression on the proliferation, invasion and apoptosis of lung cancer cells and its mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(8): 632-638.
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Abstract Objective: To investigate the effect of lncRNA TTN-AS1 on the proliferation, invasion and apoptosis of lung cancer cells by regulating miR-1271 expression. Methods: Human lung cancer A549 cells were transfected with TTN-AS1 siRNA or miR-1271 mimics. QRT-PCR was used to detect the expression of TTNAS1 and miR-1271, CCK-8 test to detect cell proliferation, Transwell test to detect cell invasion, flow cytometry to detect cell apoptosis and Western blot to detect the expression of PI3K, p-AKT, PCNA, E-cadherin and cleaved caspase 3. miR-1271 inhibitors and TTN-AS1 siRNA were co-transfected and the effect of miR-1271 inhibition on the proliferation, invasion and apoptosis of A549 cells induced by TTN-AS1 was observed. The dual luciferase reporter gene experiment was performed to detect the targeting effect of TTN-AS1/miR-1271 and miR-1271/PDK1. The effect of over-expression or inhibition of TTN-AS1/miR-1271 on PDK1 expression was analyzed by Western blot. Results: Inhibition of TTN-AS1 and over-expression of miR-1271 could significantly inhibit the proliferation and invasion of A549 cells, promote apoptosis, down-regulate the expression of PI3K, p-AKT and PCNA, and up-regulate the expression of E-cadherin and cleaved caspase 3 (P<0.05). Inhibition of miR-1271 could reverse the inhibitory effect of TTN-AS1 on the proliferation, invasion of A549 cells and the promotion of apoptosis as well. Both TTN-AS1 and PDK1 had a targeted regulatory relationship with miR-1271. Conclusion:lncRNA TTN-AS1 can inhibit the proliferation and invasion of A549 cells and promote apoptosis by targeting miR-1271/PDK1 and inhibiting PI3K/AKT signaling pathway.
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Received: 20 April 2021
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