雌二醇对小鼠急性心肌缺血再灌注损伤的保护作用及其机制

doi:10.3969/j.issn.2095-9400.2021.07.005

Abstract
Figure/Table
References (21)
Related Citation (15)

Download: PDF (2292 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the protective effect of estradiol on myocardial ischemia-reperfusion injury in mice and its possible mechanism. Methods: B-ultrasonography and myocardial TTC staining were used to study the effect of estradiol on the acute stage of myocardial ischemia-reperfusion model in mice, and protein western blot was used to detect the effect of estradiol on macrophage migration inhibitory factor (MIF) and downstream AMPK and Acetyl CoA carboxylase (ACC) phosphorylation. Results: The left ventricular ejection fraction and short-axis shortening rate of mice after myocardial ischemia-reperfusion injury under estradiol were improved, compared with other groups (P<0.05). MIF level and AMPK and ACC phosphorylation were increased in ischemia-reperfusion myocardium after estradiol treatment (P<0.05). Conclusion: Estradiol may play a protective role in the acute stage of myocardial ischemia-reperfusion injury in mice through the MIF-AMPK signaling pathway.

Key words： estradiol ischemia-reperfusion injury macrophage migration inhibitory factor adenosine monophosphate activated protein kinase mice

Received: 16 October 2020

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	QIAN Lu
	LI Zhuoyuan
	ZHANG Suqin

URL:

https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2021.07.005 OR https://xb.wmu.edu.cn/EN/Y2021/V51/I7/548

[1] MA H, WANG J, THOMAS D P, et al. Impaired macrophage migration inhibitory factor-AMP-activated protein kinase activation and ischemic recovery in the senescent heart[J].Circulation, 2010, 122(3): 282-292.
[2] MILLER E J, LI J, LENG L, et al. Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart[J]. Nature, 2008, 451(7178): 578-582.
[3] LUBETSKY J B, DIOS A, HAN J, et al. The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents[J]. J Biol Chem, 2002, 277(28): 24976-24982.
[4] ZHANG X, MU L. Association between macrophage migration inhibitory factor in the endometrium and estrogen in endometriosis[J]. Exp Ther Med, 2015, 10(2): 787-791.
[5] VEILLAT V, SENGERS V, METZ C N, et al. Macrophage migration inhibitory factor is involved in a positive feedback loop increasing aromatase expression in endometriosis[J].Am J Pathol, 2012, 181(3): 917-927.
[6] ASHCROFT G S, MILLS S J, LEI K, et al. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor[J]. J Clin Invest,2003, 111(9): 1309-1318.
[7] TUNSTALL-PEDOE H, KUULASMAA K, MAHONENM, et al. Contribution of trends in survival and coronaryevent rates to changes in coronary heart disease mortality:10-year results from 37 WHO MONICA project populations.Monitoring trends and determinants in cardiovascular disease[J]. Lancet, 1999, 353(9164): 1547-1557.
[8] LERNER D J, KANNEL W B. Patterns of coronary heart disease morbidity and mortality in the sexes: a 26-year follow-up of the Framingham population[J]. Am Heart J,1986, 111(2): 383-390.
[9] HARMAN S M, BRINTON E A, CEDARS M, et al.KEEPS: The kronos early estrogen prevention study[J].Climacteric, 2005, 8(1): 3-12.
[10] ROSSOUW J E, PRENTICE R L, MANSON J E, et al.Postmenopausal hormone therapy and risk of cardiovascular disease by age and years since menopause[J]. JAMA, 2007,297(13): 1465-1477.
[11] SALPETER S R, WALSH J M, GREYBER E, et al. Brief report: Coronary heart disease events associated with hormone therapy in younger and older women. A metaanalysis[J]. J Gen Intern Med, 2006, 21(4): 363-366.
[12] SMITH P J, ORNATSKY O, STEWART D J, et al. Effects of estrogen replacement on infarct size, cardiac remodeling,and the endothelin system after myocardial infarction in ovariectomized rats[J]. Circulation, 2000, 102(24): 2983-2989.
[13] WANG M, SMITH K, YU Q, et al. Mitochondrial connexin 43 in sex-dependent myocardial responses and estrogenmediated cardiac protection following acute ischemia/reperfusion injury[J]. Basic Res Cardiol, 2019, 115(1): 1.
[14] YANG Y, WANG I W, TURRENTINE M, et al. Postischemic application of estrogen ameliorates myocardial damage in an in vivo mouse model[J]. J Surg Res, 2018, 231: 366-372.
[15] YUAN Z, KANG L, WANG Z, et al. 17β-estradiol promotes recovery after myocardial infarction by enhancing homing and angiogenic capacity of bone marrow-derived endothelial progenitor cells through ERα-SDF-1/CXCR4 crosstalking[J]. Acta Biochim Biophys Sin (Shanghai), 2018, 50(12):1247-1256.
[16] ASHCROFT G S, MILLS S J, LEI K, et al. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor[J]. J Clin Invest,2003, 111(9): 1309-1318.
[17] LENG L, BUCALA R. Insight into the biology of macrophage migration inhibitory factor (MIF) revealed by the cloning of its cell surface receptor[J]. Cell Res, 2006,16(2): 162-168.
[18] WANG J, TONG C, YAN X, et al. Limiting cardiac ischemic injury by pharmacological augmentation of macrophage migration inhibitory factor-AMP-activated protein kinase signal transduction[J]. Circulation, 2013, 128(3): 225-236.
[19] DAYAWANSA N H, GAO X M, WHITE D A, et al. Role of MIF in myocardial ischaemia and infarction: insight from recent clinical and experimental findings[J]. Clin Sci (Lond),2014, 127(3): 149-161.
[20] JI K, WANG X, LI J, et al. Macrophage migration inhibitory factor polymorphism is associated with susceptibility to inflammatory coronary heart disease[J]. Biomed Res Int,2015, 2015: 315174.
[21] M O H A N K U M A R S , B A L A S U B R A M A N I A N P,SUBRAMANIAN M, et al. Chronic estradiol exposureharmful effects on behavior, cardiovascular and reproductive functions[J]. Reproduction, 2018, 156(5): R169-R186.