查尔酮类似物清除DPPH自由基和保护H2O2诱导的PC12细胞损伤的抗氧化活性

doi:10.3969/j.issn.2095-9400.2021.07.004

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Abstract Objective: To investigate the antioxidant activity of the synthesized chalcone analogues in scavenging DPPH free radical and protecting against H2O2-induced PC12 cell injury. Methods: PC12 cells were divided as control group (DMSO group), injury group (H2O2 group), chalcone analogue+H2O2 group and positive control group (quercetin+H2O2 group). Sixteen chalcone analogues were designed and synthesized. DPPH assay was used to detect free radical scavenging activity; MTT assay was used to detect cell activity; MDA kit was used to detect lipid peroxidation; Hoechst staining was used to detect cell apoptosis; Western blot was used to detect cleaved-caspase-3, Bcl-2 and Bax protein expression. Results: Antioxidant 13 with excellent activity and low toxicity was obtained. Compared with DMSO group, the cell survival rate and Bcl-2 protein expression were decreased significantly, but cleaved-caspase-3 and Bax protein expression, MDA content were increased significantly in H2O2 group (all P<0.05); Compared with H2O2 group, cell survival rate was increased, Bcl-2 protein expression was up-regulated, cleaved-caspase-3 and Bax expression was down-regulated, MDA level were decreased in a dose-dependent manner by group 13 treatment (all P<0.05). Conclusion: The novel chalcone analogue 13 can inhibit H2O2-induced PC12 cells apoptosis, which has good antioxidant activity.

Key words： chalcone analogues DPPH free radical hydrogen peroxide PC12 cells apoptosis

Received: 25 January 2021

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	HUANG Lili
	CHEN Chanchan
	WANG Jiabing
	ZHANG Jiafeng
	WU Jianzhang

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2021.07.004 OR https://xb.wmu.edu.cn/EN/Y2021/V51/I7/542

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