查耳酮衍生物L2H24对H2O2诱导损伤的心肌细胞的保护作用

doi:10.3969/j.issn.2095-9400.2021.07.003

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Abstract Objective: To investigate the anti-oxidative protective effect of chalcone derivative L2H24 on H2O2-induced H9c2 cardiomyocytes injury and its related molecular mechanism. Methods: The survival rate of cardiomyocytes was detected by MTT experiment; cell proliferation ability was detected by colony cloning experiment; cell pyroptosis was observed under the inverted microscope; MDA level of lipid peroxidation in cells was detected by thiobarbituric acid color reaction; the expression level of HO-1, Nrf2, Caspase-1 p48, Caspase-1 p20 and IL-1β in the cells was measured by Western blotting analysis. Results: L2H24 not only increased the survival rate of H9c2 cells damaged by H2O2 (P<0.05), promote the formation of cell colonies and reduce the level of cell lipid peroxidation (P<0.05), but also reduced the expression level of Caspase-1 p48, Caspase-1 p20 and IL-1β in cells (P<0.05) and improved the pyrolysis. Furthermore, L2H24 promoted the translocation of Nrf2 into the nucleus and up-regulated the expression level of its downstream antioxidant protein HO-1, which protected cardiomyocytes from H2O2-induced oxidative damage. Conclusion: Chalcone derivative L2H24 plays a protective role against oxidative damage, and its mechanism may be related to the activation of Nrf2/HO-1 signaling pathway.

Key words： chalcone derivatives cardiomyocytes oxidative stress antioxidant protection

Received: 20 January 2021

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	HU Linya
	HONG Chenglyu
	CHEN Qian
	HE Wenfei
	WU Jianzhang

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2021.07.003 OR https://xb.wmu.edu.cn/EN/Y2021/V51/I7/534

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