PRKG1在前列腺癌中的表达及其机制

doi:10.3969/j.issn.2095-9400.2021.06.005

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Abstract Objective: To investigate the expression of PRKG1 in prostate cancer and its mechanism by knockout of prostate cancer cell line 22RV1. Methods: The expression of PRKG1 was detected by Western blot. The PRKG1 gene in prostate cancer cell line 22RV1 was knocked out by CRISPR/Cas9 and then its effect on proliferation, migration and invasion was observed and the related apoptotic protein was detected by Western blot. Results: The positive rate of PRKG1 in prostate cancer tissue was lower (42.7% vs. 13.3%, P<0.05), compared with normal prostate tissue. There was no significant difference between the two groups in clinical stage and Gleason score (P>0.05). Knockout of PRKG1 gene significantly enhanced the proliferation, migration and invasion of prostatic cacer cells (P<0.05). PRKG1 knockout significantly decreased the levels of Ezrin, MMP9, CDH-1 and Bax (P<0.05), but increased the levels of CDH-2 and Bcl-2 (P<0.05). Conclusion: PRKG1 can inhibit the development of prostate cancer through MMP9, CDH-2, Bax, Bcl-2 and CDH-1.

Key words： prostatic neoplasm cyclic GMP-dependent protein kinase type I gene knockout

Received: 03 January 2021

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	RAO Dapang
	XIANG Zhiyang
	YU Haifeng

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2021.06.005 OR https://xb.wmu.edu.cn/EN/Y2021/V51/I6/454

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