受甲基化调控的lncRNA在乳腺癌中的表达谱及意义

doi:10.3969/j.issn.2095-9400.2021.04.003

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Abstract Objective: To study the differential expression of lncRNA between demethylated breast cancer cells and their parental lines, and to screen lncRNA from differential expression profile, so as to explore the role of lncRNA regulated by DNA promoter methylation in the occurrence and development of breast cancer. Methods: After demethylation of breast cancer BT474 cell line, the expression profiles of lncRNA in demethylation group and parental group were detected by high-throughput lncRNA microarray technology, and the expression of lncRNA in breast cancer cell line BT474, MB231, BT549 and cancer tissue were detected by qRT-PCR technology.
Meanwhile, the gene methylation sites were searched by bisulfite sequencing PCR. Results: LncRNA expression profiles of demethylated and parental cells have significantly changed. The results of quantitative RT-PCR and microarray were basically consistent. Quantitative RT-PCR showed that the expression of lncRNA uc003lxs and uc002btf in breast cancer tissues and breast cancer cell lines were higher than that in normal breast epithelial cell lines (P<0.01), and the expression of lncRNA uc003lxs and uc002btf in breast cancer tissues was higher than that in adjacent tissues (P<0.01). Compared with normal breast epithelial cell lines, the DNA methylation levels of lncRNA uc003lxs and uc002btf in breast cancer tissues and breast cancer cell lines were significantly decreased by BSP method (P<0.01); compared with adjacent tissues, the DNA methylation levels of lncRNA uc003lxs and uc002btf in breast cancer tissues were significantly decreased (P<0.01). Conclusion: The expression of lncRNA in breast cancer cell lines after demethylation treatment has been found to be significantly changed, and lncRNA uc003lxs and uc002btf have been screened out, which lays a preliminary research foundation for the study of methylation regulated lncRNA in the regulation of the occurrence and development of breast cancer.

Key words： breast cancer methylation lncRNA lncRNA microarray

Received: 21 September 2020

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	HU Qian
	LAN Xiaoshan
	LIN Yingxin
	CHEN Xiuyun
	DING Linxiaoxiao
	PANG Danmei

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2021.04.003 OR https://xb.wmu.edu.cn/EN/Y2021/V51/I4/271

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