|
|
|
| Application of therapeutic drug monitoring of vancomycin in ICU patients |
1.Department of Pharmacy, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035
|
|
| Cite this article: |
|
JIN Hui1,ZHOU Ziye1,ZHANG Xiuhua1, et al. Application of therapeutic drug monitoring of vancomycin in ICU patients[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(10): 748-751.
|
|
|
Abstract Objective: To establish a HPLC method for fast determination of vancomycin in serum and using the method for monitoring ICU patients’ therapeutic drug concentration. Methods: HPLC analysis was performed using a Water’ Xbridge column (4.6 mm×150 mm, 5 µm). The mobile phase was KH2PO4 (25 mmol•
L-1)-methanol (86:14, pH=2.4). The flow-rate was 1.0 mL•min-1 and the detection wave length was 236 nm by UV. The injection volume was 20 µL and the temperature of column was 40 ℃. Results: The concentration range of vancomycin was 1~100 mg•L-1 while the calibration curve showed good linearity (r=0.9992), and the minimum limit of quantitation was 1.0 mg•L-1. The relative standard deviations (RSD) of intra-day and inter-day were 5.83% and 6.06%, the extraction recovery was 79.71%. Twelve patients first blood valley concentration of vancomycin was between 10 and 20 mg•L-1. There were 17 cases of the treatment for effective, total effective rate was 60.71%. Conclusion: The method is simple, sensitive, exact, rapid and good repeatability. It can be used for detecting the concentration of vancomycin in ICU patients. There is bigger individual difference in blood concentration after giving Vancomycin. Clinical need monitoring results formulate reasonable individualized dosage regimen for ICU patients.
|
|
Received: 08 April 2014
|
| |
|
|
|
[1]张弋, 吴瑛, 沈中阳. 万古霉素在我院肝移植患者中的治疗药物监测及临床应用分析[J]. 中国药房, 2011, 22(26): 2451-2453.
[2]徐晓卫, 潘柔和, 胡卢丰. 肺部感染患者血清中万古霉素浓度的监测[J]. 中国临床药理学杂志, 2013, 29(6): 466-468.
[3]周秀萍, 杨长顺, 李争鸣, 等. 重症监护室MRSA耐药性及RAPD分析[J]. 中国卫生检验杂志, 2012, 22(3): 509-511.
[4]黄仲义, 肖永红, 张菁. 万古霉素临床应用中国专家共识(2011版)[J]. 中国新药与临床杂志, 2011, 30(8): 561-573.
[5]祝文兵, 阳利龙, 何周康, 等. 高效液相色谱法测定血浆中万古霉素的浓度[J]. 药物分析杂志, 2006, 26(8): 1106-1108.
[6]林秀丽, 朱金平, 费燕. HPLC法快速测定万古霉素和去甲万古霉素的血药浓度[J]. 中国临床药学杂志, 2011, 20(4):231-234.
[7]Diana J, Visky D, Roets E, et a1. Development and valida-tion of an improved method for the analysis of vancomycin by liquid chromatography selectivity of reversed-phase col-umns towards vancomycin components[J]. J Chromatogr A, 2003, 996(1-2): 115-131.
[8]范捷, 郭志磊, 于洋, 等. 高效液相色谱法测定万古霉素血药浓度[J]. 中国医院药学杂志, 2012, 32(19): 1590-1592.
[9]Cheng C, Liu S, Xiao D, et a1. LCMS/MS method development and validation for the determination of polymyxins and vancomycin in rat plsama[J]. J Chromatogr B, 2010, 878(10): 2831-2838.
[10]房德敏, 冯鑫, 陈迪, 等. 高效液相色谱法测定人血浆中万古霉素浓度[J]. 中国新药杂志, 2005, 14(5): 596-598.
[11]卢岩, 孙健, 张静, 等. 血清万古霉素浓度监测与个体化给药[J]. 医药导报, 2007, 26(3): 380-309. |
|
|
|
2014, Vol. 44 
