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| Differential expression profiles and functional prediction of circRNAs in ox-LDL stimulated macrophages |
| YE Bozhi1, LIANG Xiaohe1, ZHAO Yihan1, XUE Qize2, HUANG Zhouqing1, CAI Xueli1 |
| 1.Department of Cardiology, the Key Lab of Cardiovascular Disease of Wenzhou, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.The Second School of Medicine, Wenzhou Medical University. Wenzhou 325035, China |
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| Cite this article: |
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YE Bozhi,LIANG Xiaohe,ZHAO Yihan, et al. Differential expression profiles and functional prediction of circRNAs in ox-LDL stimulated macrophages[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2021, 51(3): 203-208.
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Abstract Objective: To investigate the differential expression profiles of circRNAs in ox-LDL-stimulated macrophages and predict its functions. Methods: Human monocyte cell line THP-1 cells were pretreated with PMA for 48 h and followed by ox-LDL (50 µg/mL) for 24 h to establish cell model. Microarray analysis was performed to investigate the differential expression profiles of circRNAs in macrophages with or without ox-LDL stimulation (as a control). qRT-PCR was utilized to verify the content variation of circRNAs in macrophages with ox-LDL stimulation. CircInteractome, TargetScan and miRecords websites were used to predict potential target genes. Results: There were 5 upregulated circRNAs and 2 downregulated circRNAs in ox-LDL-stimulated macrophages group, compared with the control group in the present study. We also verified that there were 4 circRNAs upregulated and 2 circRNAs downregulated in macrophages with ox-LDL stimulation by qRT-PCR. Bioinformatics analysis speculated that circRNA_0007478 could affect the expression of downstream proteins by binding to miR-765. Conclusion: There exist differential expressions of circRNAs in ox-LDL-stimulated macrophages. CircRNA_0007478 is predicted to be involved in the atherosclerosis process by binding to miRNA-765 to regulate downstream genes.
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Received: 13 August 2020
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