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| Leukemia inhibitory factor regulating cell activity of non-small cell lung cancer through the JAK1/STAT3 signaling pathway |
| HUANG Kefeng, JIANG Mingjun, WANG Huaying, QI Saichun. |
| Department of Cardiothoracic Surgery, the Affiliated People’s Hospital Ningbo University School of Medicine, Ningbo 315040,China |
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| Cite this article: |
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HUANG Kefeng,JIANG Mingjun,WANG Huaying, et al. Leukemia inhibitory factor regulating cell activity of non-small cell lung cancer through the JAK1/STAT3 signaling pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(12): 970-973.
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Abstract Objective: To investigate the effect of leukemia inhibitor factor (LIF) on non-small cell lung cancer (NSCLC) and its possible mechanism. Methods: Immunohistochemical, fluorescent quantitative PCR and Western blot were used to detect the expression of LIF in non-small cell lung cancer patients paracancer tissue and tumor tissue. The effect of LIF on the proliferation and invasion of tumor cell A549 cells was detected by CCK-8 and Transwell chamber. Western blot was used to detect the expression of JAK1/STAT3 signaling pathway protein. Results: The expression of LIF in cancer tissues of NSCLC was significantly higher than that
of paracancer tissues (P<0.01). After co-incubation with LIF, the proliferation and invasion ability of tumor cells were significantly enhanced (P<0.05). Western blot showed that tumor cells JAK1 and STAT3 phosphorylation levels were significantly increased (P<0.05). Conclusion: LIF expression is increased in NSCLC. The increased LIF can enhance the proliferation and invasion ability of tumor cells, which may be mediated through the JAK1/STAT3 signaling pathway.
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Received: 09 June 2020
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