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| Cryptotanshinone enhances the chemosensitivity of gemcitabine in cholangiocarcinoma |
| ZHOU Gongting,WANG Jisheng, Chen Yiming, HONG Zhong, Liao Yi. |
| Department of Hepatobiliary Surgery, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China |
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| Cite this article: |
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ZHOU Gongting,WANG Jisheng,Chen Yiming, et al. Cryptotanshinone enhances the chemosensitivity of gemcitabine in cholangiocarcinoma[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(10): 805-809.
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Abstract Objective: To investigate the effect and mechanism of cryptotanshinone on the chemosensitivity of QBC939 cells to gemcitabine. Methods: The human cholangiocarcinoma QBC939 cells were divided into four groups: control group, cryptotanshinone group, gemcitabine group and cryptotanshinone+gemcitabine group.
CCK-8 method was used to detect the inhibition rate of cell growth. Apoptosis was detected by flow cytometry.Western blot was used to detect the expression of apoptosis related protein. Results: After 24 h treatment, when the final concentration of cryptotanshinone was 10, 30 and 50 μmol/L, the inhibition rate of cell growth was
22.30%±2.15%, 42.53%±3.62% and 51.85%±4.81% respectively, which was lower than that of the control group(99.22%±3.52%), with statistical difference (P<0.05). After 24 h treatment, the apoptosis rate of cryptotanshinone+gemcitabine combination group was 35.40%±1.57%, which was higher than the cryptotanshinone group
(19.80%±0.92%), the gemcitabine group (24.20%±0.56%) and the control group (8.84%±0.58%). The difference was statistically significant (P<0.05). Western blot showed that the expression of active caspase-3 and Bax protein in the combination of cryptotanshinone and gemcitabine group was higher than that in gemcitabine group,cryptotanshinone group and control group. Conclusion: Cryptotanshinone can increase the chemosensitivity of gemcitabine in cholangiocarcinoma cells.
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Received: 12 January 2020
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