黄芩素通过烟酸受体抑制胃癌细胞增殖

doi:10.3969/j.issn.2095-9400.2020.09.011

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Abstract Objective: To investigate the function and molecular mechanisms of baicalein in inhibiting the proliferation of gastric cancer cells. Methods: CCK-8 assay was used to detect the baicalein at different concentrations and its effect on the proliferation of gastric cancer cells (MGC80-3, HGC-27, and BGC-823), and the effect of nicotinic acid receptor (GPR109A) overexpression on the viability of gastric cancer cells. Transwell migration and invasion assay was used to detect the effects of baicalein at different concentrations on the migration and invasion of gastric cancer cells in vitro. RNA interference assay was used to detect the effect of silencing GPR109A expression on the proliferation of gastric cancer cells inhibited by baicalein. Results: Baicalein significantly inhibited proliferation, migration and invasion of gastric cancer cells in vitro (P<0.05). Overexpression of GPR109A also markedly inhibited proliferation of gastric cancer cells (P<0.05). Moreover, the expression of GPR109A silenced by RNAi could block the inhibitory effect of baicalein on the proliferation of gastric cancer cells (P<0.05). Conclusion: Baicalein can significantly suppress the proliferation of gastric cancer cells in vitro. The inhibitory effect of baicalein may be attributed to the upregulation of GPR109A protein expression in gastric cancer cells.

Key words： Baicalein nicotinic acid receptor gastric cancer cell proliferation

Received: 11 October 2019

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	HUA Wenfeng
	LI Jie
	YAN Fang
	YANG Huawen
	ZHANG Zhi
	CAO Donglin

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2020.09.011 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I9/742

[1] CHEN W, ZHENG R, BAADE P D, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2): 115-132.
[2] ORDITURA M, GALIZIA G, SFORZA V, et al. Treatment of gastric cancer[J]. World J Gastroenterol, 2014, 20(7): 1635-1649.
[3] 林才志, 胡乃强, 张林英, 等. 胃癌的中医药研究进展[J].广西中医药大学学报, 2017, 20(2): 72-74.
[4] 刘沈林. 中医药治疗胃癌临床述评[J]. 江苏中医药, 2019, 51(4): 1-5.
[5] 侯文沛, 杨燕. 中医药治疗中晚期胃癌的优势与疗效机制[J]. 现代中西医结合杂志, 2019, 28(6): 677-680.
[6] 国家药典委员会. 中华人民共和国药典[M]. 2015版. 北京:中国医药科技出版社, 2015.
[7] 辛文妤, 宋俊科, 何国荣, 等. 黄芩素和黄芩苷的药理作用及机制研究进展[J]. 中国新药杂志, 2013, 22(6): 647-659.
[8] 孔晋亮, 陈一强, 闫萍, 等. 黄芩水煎液对铜绿假单胞菌生物被膜清除作用的体外研究[J]. 中华结核和呼吸杂志, 2006, 29(5): 347-348.
[9] 刘更祥. 黄芩治痢汤治疗菌痢78例疗效观察[J]. 现代中西医结合杂志, 2001, 10(5): 427.
[10] Taiming L, Xuehua J. Investigation of the absorption mechanisms of baicalin and baicalein in rats[J]. J Pharm Sci, 2006, 95(6): 1326-1333.
[11] CHEN Y, CHEN L, HONG D, et al. Baicalein inhibits fibronectin-induced epithelial-mesenchymal transition by decreasing activation and upregulation of calpain-2[J]. Cell Death Dis, 2019, 10(5): 341.
[12] MA C, XU T, SUN X, et al. Network pharmacology and bioinformatics approach reveals the therapeutic mechanism of action of baicalein in hepatocellular carcinoma[J]. Evid Based Complement Alternat Med, 2019, 2019: 7518374.
[13] KOH S Y, MOON J Y, UNNO T, et al. Baicalein suppresses stem cell-like characteristics in radio- and chemoresistant MDA-MB-231 human breast cancer cells through up-regulation of IFIT2[J]. Nutrients, 2019, 11(3): E624.
[14] YANG Y, LIU K, YANG L, et al. Bladder cancer cell viability inhibition and apoptosis induction by baicalein through targeting the expression of anti-apoptotic genes[J]. Saudi J Biol Sci, 2018, 25(7): 1478-1482.
[15] WANG C Z, ZHANG C F, CHEN L, et al. Colon cancer chemopreventive effects of baicalein, an active enteric microbiome metabolite from baicalin[J]. Int J Oncol, 2015, 47(5): 1749-1758.
[16] CHEN F, ZHUANG M, PENG J, et al. Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway[J]. Mol Med Rep, 2014, 10(4): 1999-2003.
[17] MU J, LIU T, JIANG L, et al. The traditional Chinese medicine Baicalein potently inhibits gastric cancer cells [J]. J Cancer, 2016, 7(4): 453-461.
[18] YAN X, RUI X, ZHANG K. Baicalein inhibits the invasion of gastric cancer cells by suppressing the activity of the p38 signaling pathway[J]. Oncol Rep, 2015, 33(2): 737-743.
[19] O'HAYRE M, DEGESE M S, GUTKIND J S. Novel insights into G protein and G protein-coupled receptor signaling in cancer[J]. Curr Opin Cell Biol, 2014, 27: 126-135.
[20] THANGARAJU M, CRESCI G A, LIU K, et al. GPR109A is a G-protein-coupled receptor for the bacterial fermentation product butyrate and functions as a tumor suppressor in colon [J]. Cancer Res, 2009, 69(7): 2826-2832.
[21] ELANGOVAN S, PATHANIA R, RAMACHANDRAN S, et al. The niacin/butyrate receptor GPR109A suppresses mammary tumorigenesis by inhibiting cell survival[J]. Cancer Res, 2014, 74(4): 1166-1178.
[22] 孙沛林, 朴日龙, 王莹, 等. 黄芩素抑制胃癌MGC-803细胞增殖和迁移[J]. 中国病理生理杂志, 2018, 34(3): 417-422.
[23] 张超贤, 韩宇, 郭李柯. 黄芩素对人胃癌SGC7901细胞迁移及侵袭的影响及其作用机制[J]. 西安交通大学学报(医学版), 2016, 37(6): 892-896, 909.
[24] 金京春, 叶晶, 蔡洙哲, 等. 血小板型12-LOX抑制剂黄芩素抑制胃癌MKN-28细胞增殖和迁移[J]. 肿瘤, 2016, 36(2): 173-180.
[25] TUNARU S, KERO J, SCHAUB A, et al. PUMA-G and HM74 are receptors for nicotinic acid and mediate its anti-lipolytic effect[J]. Nat Med, 2003, 9(3): 352-355.
[26] 黄燕, 刘培庆. 烟酸受体GPR109A介导的烟酸作用机制研究进展[J]. 中国药理学与毒理学杂志, 2012, 26(1): 105-107.
[27] GRAFF E C, FANG H, WANDERS D, et al. Anti-inflammatory effects of the hydroxycarboxylic acid receptor 2[J]. Metabolism, 2016, 65(2): 102-113.
[28] WESSLER S, KRISCH L M, ELMER D P, et al. From inflammation to gastric cancer-the importance of Hedgehog/GLI signaling in Helicobacter pylori-induced chronic inflammatory and neoplastic diseases[J]. Cell Commun Signal, 2017, 15(1):15.