具有抗细菌黏附和杀菌双重功能的(HA/CHI-Van)5涂层的制备

doi:10.3969/j.issn.2095-9400.2020.09.004

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Abstract Objective: To study an implant coated with a (HA/CHI-Van)5 multilayer membrane structure to prevent implant infection. Methods: The HA and CHI-Van solution were assembled to prepare a (HA/CHI-Van)5 multilayer film structure, and the assembly process was detected by zeta potential. The antibacterial adhesion function of the multilayer film was evaluated by a live bacterial staining test. And in vitro antibacterial effect was also tested by jailbreak experiments and FE-SEM experiment. Most importantly, adequate animal experiments were designed besides X-ray, Micro-CT and bone tissue sections were to discuss in vivo experiments. Results: Each time a different monolayer was assembled, the zeta potential alternated, demonstrating the successful assembly of the multilayer film. Live bacteria staining experiments showed that CHI in the multilayer membrane structure had a certain anti-bacterial adhesion effect. The jailbreak experiment and the FE-SEM experiment proved that the (HA/CHI-Van)5 multilayer membrane structure had a good antibacterial effect in vitro. The X-ray score of (HA/CHI-Van)5 group was 0.7, while the score of blank group was 12.3. (HA/CHI-Van)5 group was superior to blank group in bone mineral density, trabecular bone number, relative bone volume, and bone tissue section, and was very close to sham operation group. Conclusion: By applying a (HA/CHI-Van)5 multilayer membrane structure in the implant surface, a certain anti-bacterial adhesion and good antibacterial effect can be obtained, and the implant infection can be prevented.

Key words： implant; infection bacterial layer-by-layer self-assembly antibiotic

Received: 05 November 2019

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	LIAO Xin
	HUANG Yixing

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2020.09.004 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I9/705

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