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| The regulatory role of RanBPM in renal tubular sodium chloride cotransporter and its effect on ERK1/2 signaling pathway |
| ZHANG Yiqian1, ZHUANG Zhizhi1, SHEN Meng1, ZHUANG Jieqiu2, CAI Hui1 |
| 1.Department of Nephrology, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.Department of Pediatric Nephrology, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China |
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| Cite this article: |
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ZHANG Yiqian,ZHUANG Zhizhi,SHEN Meng, et al. The regulatory role of RanBPM in renal tubular sodium chloride cotransporter and its effect on ERK1/2 signaling pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(7): 553-556，562.
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Abstract Objective: To observe the effect of Ran binding protein M (RanBPM) on the expression of NCC protein in renal tubules and the change of ERK1/2 signaling pathway protein. Methods: Immunocoprecipitation was used to detect whether there was direct interaction between RanBPM and WNK4 protein. The effect of transfection of RanBPM plasmid on ERK1/2 phosphorylation induced by EGF was observed. RanBPM gene was inhibited by overexpression of RanBPM protein or siRNA. The content of endogenous NCC protein and the level of ERK1/2 phosphorylation were detected by Western blot. The effect of RanBPM on WNK4 regulation of NCC by ERK1/2 phosphorylation was explored before and after transfection. Results: Immunocoprecipitation confirmed that there was a direct interaction between WNK4 and RanBPM. RanBPM could inhibit EGF induced ERK1/2 phosphorylation. Overexpression of RanBPM reduced ERK1/2 phosphorylation (1.000±0.074 vs. 0.275± 0.041, P<0.01) and increased NCC protein expression (1.000±0.115 vs. 1.470±0.105, P<0.01). After siRNA silenced RanBPM gene, ERK1/2 phosphorylation increased (1.000±0.194 vs. 2.301±0.220, P<0.01), resulting in decreased NCC protein expression (1.000±0.223 vs. 0.556±0.132, P<0.01). When RanBPM was transfected, WNK4 inhibited NCC protein expression and no longer affected ERK1/2 phosphorylation. Conclusion: RanBPM interacted with WNK4 affects the regulation of ERK1/2 signaling pathway in NCC protein expression.
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