RSK2通过调控人皮肤成纤维细胞的增殖与迁移促进皮肤创面修复

doi:10.3969/j.issn.2095-9400.2020.07.001

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Abstract Objective: To investigate the effect of RSK2 on skin wound healing and its mechanism. Methods: Western blot and immunofluorescence staining were used to detect the expression of RSK2 under wound conditions. Scratch test, CCK-8 detection and Western blot were used to investigate the role of RSK2 inhibitor Bix 02565 on human primary dermal fibroblasts (HDF) at the cellular level. In vivo, HE staining, Masson staining and immunofluorescence staining were used to detect the effect of RSK2 inhibitor on skin wound repair. Results: Western blot and immunofluorescence staining showed that the skin trauma modal did not affect the total protein expression of RSK2 in fibroblasts, while the use of Bix 02565 to inhibit the activity of RSK2 in vitro could significantly inhibit the proliferation and migration of fibroblasts, and inhibit the expression of G1/S-specific cyclin-D1 and Fibronectin. In vivo, the inhibitor restrained proliferating cell nuclear antigen (PCNA) and collagen fibers production of fibroblasts, inhibited platelet-endothelial cell adhesion molecule (CD31), blocked vessels regeneration and delayed the repair rate of wound healing in mice. Conclusion: RSK2 facilitates skin wound healing mainly by promoting the proliferation and migration of fibroblasts and via angiogenesis.

Key words： RSK2 Bix 02565 fibroblast wound healing

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	WANG Jia’nan
	ZHU Zhongxin
	CONG Weitao

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2020.07.001 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I7/517

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