多囊卵巢综合征伴胰岛素抵抗患者血清miRNA差异表达及二甲双胍干预作用

doi:10.3969/j.issn.2095-9400.2020.06.007

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Abstract Objective: To study the effect of metformin on the serum gene expression profiles in polycystic ovary syndrome (PCOS) patients with insulin resistance (IR). Methods: Six PCOS-IR patients were selected for the metformin treatment group and six healthy women of childbearing age as the control group. Gene chip and RT-PCR were used to study the changes of serum miRNA expression in clinically diagnosed PCOS-IR patients and the changes of serum miRNA expression after metformin intervention. For miRNAs with differential expression after screening and validation, bioinformatics method was used before gene ontology and KEGG pathway databases were used for function and pathway analysis. Results: The results of cluster analysis showed that there were differences between the untreated PCOS-IR group and the healthy control group as well as before and after metformin treatment in PCOS-IR patients. RT-PCR validated that there were 10 differentially expressed miRNAs in the untreated PCOS-IR group compared to the healthy control group, and 8 differentially expressed miRNAs before and after metformin treatment in PCOS-IR patients. GO analysis showed that the differentially expressed genes were related to transcription regulation, signal transduction, transmembrane transport, cell proliferation, cell apoptosis, cell metabolism, cell adhesion, protein phosphorylation, protein transport, multicellular biological development, coagulation function, nerve conduction, etc. KEGG analysis showed that the differentially expressed miRNAs were significantly involved in the following pathways: EGFR tyrosine kinase inhibitor resistance, Rap1 signaling pathway, Wnt signaling pathway, prostate cancer pathway, mTOR signaling pathway, cAMP signaling pathway, phosphatidylinositol signaling system, MAPK signaling pathway. With this method to narrow the search scope, 878 genes were obtained and related to insulin resistance and metformin therapy in PCOS patients. Conclusion: Some miRNAs were suggested to be involved in the pathogenesis of insulin resistance in PCOS patients and the treatment process of metformin.

Key words： polycystic ovary syndrome insulin resistance serum miRNA metformin

Received: 10 September 2019

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	WANG Chenye
	DING Caifei

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2020.06.007 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I6/465

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