血管内皮生长因子基因多态性与子宫内膜异位症的相关性

doi:10.3969/j.issn.2095-9400.2020.05.008

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Abstract Objective: To investigate whether polymorphisms of vascular endothelial growth factor (VEGF) in promoter region (rs3025040 C/T, rs10434 G/A) are associated with hereditary susceptibility of endometriosis. Methods: A total of 150 patients with endometriosis pathologically diagnosed who were admitted to the First Affiliated Hospital of Wenzhou Medical University from March 2014 to March 2015 were selected as case group and another 150 healthy volunteers were collected as control group. 5 mL blood samples were obtained from both groups in this assay and genomic DNA was extracted from peripheral blood leukocyte using TIANamp Blood DNA Kit. Special DNA PCR primers were designed using Primer Premier 5 software. The genotypes of all sites were detected by polymorphism chain reaction and restriction fragment length polymorphism analysis (PCRRFLP). Results: The C and T allele frequencies in VEGF-rs3025040 C/T site in case group and control group were 76.67%, 23.33% and 58.00%, 42.00%, respectively. There was significant difference in allele distributions of the VEGF-rs3025040 C/T site between two groups (χ2=23.762, P<0.001, OR=1.800, 95%CI=1.410-2.298). Compared with the T allele, the C allele significantly increased the risk of developing endometriosis. The G and A allele frequency in VEGF-rs10434 G/A site in case group and control group was 60.00%, 40.00% and 81.67%, 18.33% respectively. There was significant difference in allele distributions of the VEGF-rs10434 G/A site between two groups (χ2=34.084, P<0.001, OR=1.344, 95%CI=1.207-1.497). Compared with the G allele, the A allele significantly increased the risk of developing endometriosis. Conclusion: The results suggest that VEGFrs3025040 C/T, rs10434 G/A polymorphisms are associated with endometriosis.

Key words： endometriosis vascular endothelial growth factor polymorphism

Received: 06 July 2019

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	GU Hangzhi
	ZHANG Xu
	WANG Guangyan
	LIN Rongrong
	HU Yan.

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https://xb.wmu.edu.cn/EN/ 10.3969/j.issn.2095-9400.2020.05.008 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I5/391

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