恒温扩增技术在男性淋球菌性尿道炎病原菌RNA检测中的应用

doi:10.3969/j.issn.2095-9400.2020.02.012

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Abstract Objective: To evaluate the application value of RNA simultaneous amplification and testing technology in the detection of male gonococcal urethritis. Methods: From June 2017 to August 2018, a total of 322 male patients with suspected gonococcal urethritis infection in Wenzhou People’s Hospital were detected by RNA simultaneous amplification and testing (RNA-SAT) and real-time fluorescence quantitative PCR (FQ-PCR), then the results were statistically analyzed. Results: The highest NG positive rate was 35.1% (113/322) by urine RNA-SAT method, and the lowest NG positive rate was 26.1% (84/322) by urine FQ-PCR method. The difference of positive rates in urine RNA-SAT, urine FQ-PCR and isolation culture was statistically significant (χ2=6.312, P<0.05). The difference of positive rates in swab RNA-SAT, swab FQ-PCR and isolation culture was not statistically significant (χ2=1.039, P>0.05). The highest sensitivity of urine RNA-SAT was 97.9% (93/95), and that of urine FQ-PCR method was 81.1% (77/95). According to ROC curve, swab RNA-SAT and urine RNA-SAT method have a relatively high diagnostic value (AUC>0.9). After 7 days of treatment, clinical symptoms in 81 patients disappeared and negative leukocyte were re-examined. The difference in negative rates in urine RNA-SAT, urine FQ-PCR and isolation culture was not statistically significant (χ2=2.531, P>0.05). The difference in negative rates of swab RNA-SAT, swab FQ-PCR and isolation culture was also not statistically significant (χ2=2.933, P>0.05). Conclusion: Both RNA-SAT and FQ-PCR can be used to diagnose NG infection; however, RNA-SAT has the advantages of convenient sampling, short time, little pollution and accurate results. So it can be used to detect NG in male gonococcal urethritis and to evaluate the effect and prognosis.

Key words： gonococcus urethritis nucleic acid amplification technology culture method

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	DAI Xianning
	TONG Yu
	LIN Jianping
	Shi Jianyou
	Chen Lu
	XU Kai

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2020.02.012 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I2/145

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