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Effect of curcumin in pulmonary hypertension rats by NLRP3/Caspase-1/IL-1β axis |
CAI Xueding, CHEN Mayun, LI Wenya, WU Peiliang, YAO Dan, HUANG Xiaoying |
Department of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China |
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Cite this article: |
CAI Xueding,CHEN Mayun,LI Wenya, et al. Effect of curcumin in pulmonary hypertension rats by NLRP3/Caspase-1/IL-1β axis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(2): 98-101,107.
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Abstract Objective: To investigate the therapeutic effect and mechanism of curcumin in hypoxia-induced pulmonary hypertension (HPH). Methods: Pulmonary artery smooth muscle cells (PASMCs) and Sprague-Daeley (SD) rats were studied in vitro and in vivo respectively. The PASMCs and SD rats were divided into 3 groups: normoxia group (N), hypoxia group (H), hypoxia+curcumin group (HC). The activity of PASMCs cells in each group was measured by cck-8, and the expressions of NLRP3, Caspase-1 and il-1β protein in PASMCs were detected by Western blot. The average pulmonary artery pressure and the degree of right cardiac hypertrophy in each group of rats were measured by hemodynamics, while the degree of pulmonary vascular remodeling in each group of rats was observed by HE staining, and the expression difference of the above-mentioned proteins in the lung homogenate in each group of rats was detected by western blot. Results: Compared with the N group, the quantity of PASMCs in H group were significantly increased. The NLRP3/Caspase-1/IL-1β protein expression in H group was increased. The mPAP and right cardiac hypertrophy index in group H were significantly increased, and the pulmonary vascular remodeling was obvious. Compared with the H group, the quantity of PASMCs in HC group were significantly decreased, and the expression of NLRP3/Caspase-1/IL-1β protein in PASMCs and lung homogenate was decreased, mPAP and right cardiac hypertrophy index were significantly decreased in HC group, and pulmonary vascular remodeling was improved. Conclusion: Curcumin could inhibits pulmonary arterial hypertension, pulmonary vascular remodeling and right ventricle hypertrophy via suppressing NLRP3/Caspase-1/IL-1β axis mediated inflammatory reaction.
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