omega-3脂肪酸在围绝经期焦虑样小鼠中的抗焦虑作用及机制

doi:10.3969/j.issn.2095-9400.2020.01.009

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Abstract Objective: To analyze the anti-anxiety effect of omega-3 polyunsaturated fatty acids (n-3 PUFA) in ovariectomized mice subject to unpredictable chronic stress and to reveal its mechanism. Methods: Chronic unpredictable stress was performed 1 week after bilateral ovarian ablation in mice to establish the model of menopausal anxiety. Using classical anxiety behavior tests (e marble-burying test and elevated plus maze test) to verify the anti-anxiety effect of chronic administration of n-3 PUFA (10, 20, 60 mg/kg) for 14 days. The mechanism of antianxiety of n-3 PUFA was investigated by HPLC method to detect the contents of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), noradrenaline (NE), dopamine (DA), dopac neurotransmitters and their metabolites. Results: Chronic administration of menopausal anxiety-like mice with n-3 PUFA (20, 60 mg/kg) effectively decreased the number of buried marbles and elevated the number and time to open arm at elevated plus maze test (P<0.05). HPLC results showed that chronic administration of menopausal anxiety-like mice with n-3 PUFA (20, 60 mg/kg) significantly affected 5-HT, 5-HIAA/5-HT, NE, DA in prefrontal cortex (P<0.05) and also significantly improved the concentration of 5-HT, 5-HIAA/5-HT, NE in hippocampus (P<0.05) and regulated the level of 5-HT, NE in hypothalamus (P<0.05). In summary, the possible mechanism of n-3 PUFA involves regulating neurotransmitters and its metabolite pathways in hippocampus, prefrontal cortex and hypothalamus. Conclusion: n-3 PUFA is an ideal drug for treating menopausal anxiety through regulating the neurotransmitters and its metabolites in hippocampus, prefrontal cortex and hypothalamic.

Key words： omega-3 fatty acids perimenopause anxiety disorders neurotransmitters mice

Received: 16 April 2019

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	HE Chujun
	TU Junxue
	HUANG Wanran
	BAO Shihui

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2020.01.009 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I1/40

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