miR-144-5p、miR-668-3p、miR-134-5p在儿童原发性免疫性血小板减少症中的表达

doi:10.3969/j.issn.2095-9400.2020.01.004

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Abstract Objective: To investigate the changes of microRNAs (miR-144-5p, miR-668-3p and miR-134-5p) in peripheral blood mononuclear cells (PBMCs) in children with primary immune thrombocytopenia (ITP). Methods: Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the relative expression of the three miRNAs from 25 ITP patients and healthy control in PBMCs. Platelet count and immature platelet fraction (IPF%) were detected by Sysmex XE-5000 automatic blood analyzer. The correlations of the miRNAs expression with peripheral platelet count were analyzed and diagnostic value was measured by receiver operating characteristic curve. Results: Compared with the normal control group, the expressions of miR-144-5p and miR-668-3p of the ITP group in PBMCs were significantly up-regulated (P<0.05), and were negatively correlated with platelet count (r=-0.802, P<0.001; r=-0.753, P<0.001), while the expressions of miR-134-5p was down-regulated significantly (P<0.05). The expressions of miR-144-5p and miR-668-3p in the acute ITP group were higher than those in the chronic ITP group (P<0.05). In terms of the single indicator, the AUC of miR-144-5p combined with IPF% in the diagnosis of acute ITP was 0.960. The sensitivity and specificity were 93.3% and 100% respectively. The AUC of miR-134-5p combined with IPF% in the diagnosis of chronic ITP was 0.967. The sensitivity and specificity were 90.0% and 93.3% respectively. Conclusion: miR-144-5p and miR-668-3p in PBMCs were significantly up-regulated and miR-134-5p was down-regulated in children with ITP, which suggests they may play an important role in the incidence and development of ITP.

Key words： immune thrombocytopenia microRNA immature platelet fraction quantitative real-time polymerase chain reaction

Received: 15 May 2019

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	JIN Susu
	LI Fanfan
	YE Manli
	SHU Kuangyi
	Li Shanshan
	LIU Jie
	JIANG Minghua

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2020.01.004 OR https://xb.wmu.edu.cn/EN/Y2020/V50/I1/19

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