RNA干扰SALL4基因对儿童睾丸卵黄囊瘤细胞增殖和凋亡的影响

doi:10.3969/j.issn.2095-9400.2019.12.006

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1704 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To study the effect of RNA interference gene SALL4 on the proliferation and apaptosis of testicular yolk sac tumor cells in children. Methods: The pGPU6/GFP/neo-sall4 shRNA was constructed as a vector to be transfected into pediatric testicular yolk sac tumor cells and interfere in SALL4. The interfering effect was verified by detecting mRNA and protein levels. CCK-8 and flow cytometry were employed to study the inhibition effect of interfering SALL4 in pediatric testicular yolk sac tumor cells. Results: After successfully transfecting the vector into pediatric testicular yolk sac tumor cells, the mRNA expression magnitudes of SALL4 interfered group was 24.0%±4.0%, as compared with Negative group (93.0%±43.0%), with lower levels of mRNA expression (P<0.05). And the protein expressions of SALL4 interfered group (0.39±0.10) had a lower protein expression than Negative group (1.02±0.09). Besides, the proliferation was significantly reduced, while the apoptosis rate was significantly increased in vitro (P<0.05). Conclusion: Targeting SALL4 RNA could inhibit the proliferation and induce apoptosis of pediatric testicular yolk sac tumor cells, suggesting that SALL4 may be a potential therapeutic target for pediatric testicular yolk sac tumor.

Key words： RNA interference SALL4 children testicular yolk sac tumor

Received: 15 July 2019

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LYU Min
	CAI Zuren
	CHEN Xiaoming

URL:

https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.12.006 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I12/885

[1] DE CELIS J F, BARRIO R. Regulation and function of Spalt proteins during animal development[J]. Int J Dev Biol, 2009, 53(8-10): 1385-1398.
[2] FIROR A E, JARES A. Nuclear localization of SALL4: a stemness transcription factor[J]. Cell Cycle, 2014, 13(10): 1522-1523.
[3] Tatetsu H, Kong N R, Chong G, et al. SALL4, the missing link between stem cells, development and cancer[J]. Gene, 2016, 584(2): 111-119.
[4] MA Y, CUI W, YANG J, et al. SALL4, a novel oncogene, is constitutively expressed in human acute myeloid leukemia (AML) and induces AML in transgenic mice[J]. Blood, 2006, 108(8): 2726-2735.
[5] LI A, JIAO Y, YONG K J, et al. SALL4 is a new target in endometrial cancer[J]. Oncogene, 2015, 34(1): 63-72.
[6] FORGHANIFARD M M, MOGHBELI M, RAEISOSSADATI R, et al. Role of SALL4 in the progression and metastasis of colorectal cancer[J]. J Biomed Sci, 2013, 20: 6.
[7] DIRICAN E, AKKIPRIK M. Functional and clinical significance of SALL4 in breast cancer[J]. Tumour Biol, 2016, 37(9): 11701-11709.
[8] KOBAYASHI D, KURIBAYASHI K, TANAKA M, et al. Overexpression of SALL4 in lung cancer and its importance in cell proliferation[J]. Oncol Rep, 2011, 26(4): 965-970.
[9] 吕敏, 张浩川, 同毓龙, 等. 婆娑双树基因4干扰载体对儿童睾丸卵黄囊瘤的转染及干扰效果评价[J]. 中华小儿外科杂志, 2016, 37(9): 673-676.
[10] ZHAO J, CHEN C, ZHANG H, et al. Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor[J]. J Transl Med, 2012, 10: 46.
[11] 张浩川, 沈金辉, 陈聪德, 等. 小儿睾丸卵黄囊瘤细胞的体外培养及生物学特性研究[J]. 中华小儿外科杂志, 2011, 32(3): 186-189.
[12] TOURNE M, RADULESCU C, ALLORY Y. Testicular germ cell tumors: Histopathological and molecular features [J]. Bull Cancer, 2019, 106(4): 328-341.
[13] CABALLERO MORA F J, MUÑOZ CALVO M T, GARCIA ROS M, et al. Testicular and paratesticular tumors during childhood and adolescence[J]. An Pediatr (Barc), 2013, 78(1): 6-13.
[14] WU D, SHEN N, LIN X, et al. Prepubertal testicular tumors in China: a 10-year experience with 67 cases[J]. Pediatr Surg Int, 2018, 34(12): 1339-1343.
[15] XIONG J, TODOROVA D, SU N Y, et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells[J]. J Cell Biol, 2015, 208(5): 513-520.
[16] OIKAWA T, KAMIYA A, KAKINUMA S, et al. Sall4 regulates cell fate decision in fetal hepatic stem/progenitor cells[J]. Gastroenterology, 2009, 136(3): 1000-1011.
[17] NICOLE L, SANAVIA T, VERONESE N, et al. Oncofetal gene SALL4 and prognosis in cancer: A systematic review with meta-analysis[J]. Oncotarget, 2017, 8(14): 22968-22979.
[18] ZHANG X, ZHANG P, SHAO M, et al. SALL4 activates TGF-beta/SMAD signaling pathway to induce EMT and promote gastric cancer metastasis[J]. Cancer Manag Res, 2018, 10: 4459-4470.
[19] YONG K J, GAO C, LIM J S, et al. Oncofetal gene SALL4 in aggressive hepatocellular carcinoma[J]. N Engl J Med, 2013, 368(24): 2266-2276.
[20] WANG F, ZHAO W, KONG N, et al. The next new target in leukemia: The embryonic stem cell gene SALL4[J]. Mol Cell Oncol, 2014, 1(4): e969169.
[21] SHEN H, LI L, WANG D, et al. Higher expression of SALL4 predicts poor cancer prognosis: A meta-analysis[J]. Cancer Biomark, 2017, 19(4): 365-373.
[22] YANG J. SALL4 as a transcriptional and epigenetic regulator in normal and leukemic hematopoiesis[J]. Biomark Res, 2018, 6: 1.
[23] LIU W, XIAO P, WU H, et al. MicroRNA-98 plays a suppressive role in non-small cell lung cancer through inhibition of SALL4 protein expression[J]. Oncol Res, 2017, 25(6): 975-988.
[24] PAN L, LIANG W, GU J, et al. Long noncoding RNA DANCR is activated by SALL4 and promotes the proliferation and invasion of gastric cancer cells[J]. Oncotarget, 2018, 9(2): 1915-1930.
[25] CAO D, HUMPHREY P A, ALLAN R W. SALL4 is a novel sensitive and specific marker for me astatic germ cell tumors, with particular utility in detection of metastatic yolk sac tumors[J]. Cancer, 2009, 115(12): 2640-2651.
[26] 李想, 王珊, 邓晓斌, 等. SALL4在儿童卵黄囊瘤中表达的研究[J]. 中华小儿外科杂志, 2011, 32(8): 585-589.
[27] GAO C, KONG N R, CHAI L. The role of stem cell factor SALL4 in leukemogenesis[J]. Crit Rev Oncog, 2011, 16(1-2): 117-127.