炎症通路在强直性脊柱炎发病中作用的转录组学研究

doi:10.3969/j.issn.2095-9400.2019.12.004

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Abstract Objective: To study the transcriptomics of ankylosing spondylitis (AS) to elucidate the key genes and pathways involved in the pathogenesis of the disease. Methods: Peripheral blood mononuclear cells from AS patients and healthy controls were collected. RNA was extracted and gene expression profiles were obtained by high-throughput RNA sequencing. Bioinformatics method was used to compare the gene expressions of AS patients and healthy controls to find differentially expressed genes (DEGs). GO function annotation was performed, and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment as well as protein interaction network (PPI) were analyzed. The levels of important cytokines involved in the pathway were further verified in the peripheral blood of AS patients and healthy controls by Enzyme-linked immunosorbent assay (ELISA). Results: We found 153 differentially expressed genes (DEGs) in AS patients and healthy controls, of which 149 genes were up-regulated and 4 genes down-regulated, and all of them were closely related to each other. The biological function of the up-regulated gene was found to be mainly related to inflammatory pathways such as TNF signaling pathway and chemokine signaling. In addition, it was confirmed in peripheral plasma that the expression levels of inflammatory cytokines IL-2, IL-6, IL-10, TNF-α and IFN-γ were significantly higher in AS patients than in healthy controls (P<0.05). Conclusion: This study reveals important inflammatory genes and signaling pathways that play a role in the pathogenesis of ankylosing spondylitis, which provides a reference for targeted anti-inflammatory therapy in AS patients.

Received: 22 May 2019

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	LI Chenlu
	FANG Jinxia
	LUO Jing
	ZHU Xiaochun
	WANG Xiaobing

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.12.004 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I12/872

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