丹酚酸B对成骨细胞氧化损伤的保护作用

doi:10.3969/j.issn.2095-9400.2019.12.003

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Abstract Objective: To explore the protective effect of salvianolic acid B (Sal B) on oxidative damage of osteoblasts. Methods: The oxidative damage model of osteoblasts induced by tert-butyl hydroperoxide (TBHP) was constructed. Cell viability was detected by MTT assay. The level of reactive oxygen species (ROS)was detected by DCFH-DA fluorescence staining. Real-time PCR was used to assess the levels of osteogenic differentiation genes. Alizarin red staining was used to detect the extracellular matrix mineralization of osteoblasts. Results: The results of MTT showed that the inhibition of osteoblast viability by TBHP was in a time- and concentration-dependent manner. Treatment with 250 μmol/L TBHP for 6 h was the half inhibitory condition for osteoblasts. Sal B significantly restored cell viability up to 84.6%, the expression of differentiation genes and extracellular matrix mineralization level of osteoblasts (P<0.001) and meanwhile decreased the level of ROS (P<0.001). Conclusion: Sal B effectively inhibited the oxidative damage and restored osteoblasts’ viability and function.

Key words： salvianolic acid B osteoblast TBHP oxidative damage differentiation

Received: 08 August 2019

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	RONG Caili
	SANG Chunhui
	CHEN Dong
	TANG Qi

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https://xb.wmu.edu.cn/EN/ 10.3969/j.issn.2095-9400.2019.12.003 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I12/867

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