雌激素β受体在人椎间盘髓核细胞上的表达

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Abstract Objective: To investigate the expression of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc. Methods: Seven nucleus pulposus specimens were obtained from 7 patients during surgery. Cultured disc cells were used for immunocytochemical localization of ERβ. mRNA was isolated from cultured human disc cells, and RT-PCR amplification of ERβ was employed to document molecular expression of this receptor. Results: Expression and localization of estrogen receptor-β were detected in all 7 specimens. A goldview-stained agarose gel electrophoresis showing gene expression of ERβ as a single band at the predicted 323 bp size. Localization was seen in the cytoplasm in all cultured disc cells with the immunocytochemistry method. Conclusion: This study demonstrates the expression of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc

Key words： intervertebral disc nucleus pulposus estrogen receptor-β

Received: 06 June 2013

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	Articles by authors
	CHEN Hongliang1
	KE Xiurong2
	LOU Chao1
	LI Youyu2
	HU Chunfei2
	GUO Caiyun2
	CHEN Sisi2
	XU Huazi1

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I9/646

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