AMPK/STAT1通路参与土木香内酯改善H2O2引起的神经细胞PC12凋亡

doi:10.3969/j.issn.2095-9400.2019.11.006

Abstract
Figure/Table
References (0)
Related Citation (3)

Download: PDF (1669 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To study the effect of alantolactone (ALA) on apoptosis of PC12 induced by hydrogen peroxide (H2O2). Methods: Cell proliferation was detected by MTT after treatment with different concentrations of ALA (1, 5, 10, 20, 50, and 100 μmol/L). After cells were pretreated with different concentrations of ALA (1 and 5 μmol/L) and then stimulated by H2O2, cell proliferation was detected by flow cytometry and MTT. The control group, H2O2 (100 μmol/L) group and H2O2 (100 μmol/L)+ALA (1 and 5 μmol/L) were established. Cell apoptosis was detected by flow cytometry, cell proliferation by MTT and phosphorylation of AMPK and STAT1 by Western blot. Phosphorylation of STAT1 was detected by Western blot after treatment with small interfering RNA (siRNA) of AMPK and ALA in PC12. Results: High concentration of ALA inhibited the proliferation of PC12 cells. Compared with the control group, the apoptosis of PC12 cells increased after stimulation by H2O2. Compared with H2O2 group, the apoptosis of PC12 cells in H2O2+ALA group decreased significantly in a dose-dependent manner. Silencing AMPK expression did not affect H2O2-induced phosphorylation of STAT1 in PC12 cells induced by H2O2. ALA was used to treat PC12 cells after silencing AMPK. Compared with siAMPK+H2O2 group, phosphorylation of STAT1 in ALA+siAMPK+H2O2 group showed no significant difference. Conclusion: ALA could regulate the phosphorylation of STAT1 in PC12 cells induced by H2O2 via activating AMPK and thus affect cell apoptosis.

Key words： alantolactone PC12 cells H2O2 AMPK STAT1

Received: 12 February 2019

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XU Jianfei
	WANG Yang
	HU Xujun
	WANG Hua
	XU Zhaojun

URL:

https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.11.006 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I11/814

[1] LIM H S, JIN S E, KIM O S, et al. Alantolactone from saussurea lappa exerts antiinflammatory effects by unhibiting chemokine production and STAT1 phosphorylation in TNF-alpha and IFN-gamma-induced in HaCaT cells[J]. Phytother Res, 2015, 29(7): 1088-1096.
[2] CHUN J, CHOI R J, KHAN S, et al. Alantolactone suppresses inducible nitric oxide synthase and cyclooxygenase-2 expression by down-regulating NF-kappaB, MAPK and AP-1 via the MyD88 signaling pathway in LPS-activated RAW 264.7 cells[J]. Int Immunopharmacol, 2012, 14(4): 375-383.
[3] WANG X, LAN Y L, XING J S, et al. Alantolactone plays neuroprotective roles in traumatic brain injury in rats via anti-inflammatory, anti-oxidative and anti-apoptosis pathways [J]. Am J Transl Res, 2018, 10(2): 368-380.
[4] CUI L, BU W, SONG J, et al. Apoptosis induction by alantolactone in breast cancer MDA-MB-231 cells through reactive oxygen species-mediated mitochondrion-dependent pathway[J]. Arch Pharm Res, 2018, 41(3): 299-313.
[5] KIM M, SONG K, KIM Y S. Alantolactone improves prolonged exposure of interleukin-6-induced skeletal muscle inflammation associated glucose intolerance and insulin resis-tance[J]. Front Pharmacol, 2017, 8: 405.
[6] SEO J Y, LIM S S, KIM J, et al. Alantolactone and isoalantolactone prevent amyloid beta25-35-induced toxicity in mouse cortical neurons and scopolamine-induced cognitive impairment in mice[J]. Phytother Res, 2017, 31(5): 801-811.
[7] 宋修云, 胡金凤, 陈乃宏. 神经细胞凋亡与脑缺血疾病[J]. 中国药理学通报, 2012, 28(3): 307-310.
[8] 杨春辉, 蔡虹, 闫江舟, 等. 土木香内酯对K562/ADR细胞增殖的抑制作用及其机制探讨[J]. 中华血液学杂志, 2014, 35(6): 515-518.
[9] 米旭光, 魏海峰, 刘磊, 等. 土木香内酯对肿瘤睾丸抗原TSP50高表达肿瘤的抑制作用研究[C]. 中国免疫学会全国免疫学学术大会, 2014.
[10] GU Y, DENNIS S M, KIERNAN M C, et al. Aerobic exercise training may improve nerve function in type 2 diabetes and pre-diabetes: a systematic review[J]. Diabetes Metab Res Rev, 2019, 35(2): e3099.
[11] MA L, MA S, HE H, et al. Perivascular fat-mediated vascular dysfunction and remodeling through the AMPK/mTOR pathway in high-fat diet-induced obese rats[J]. Hypertens Res, 2010, 33(5): 446-453.
[12] HE C, LI H, VIOLLET B, et al. AMPK suppresses vascular inflammation in vivo by inhibiting signal transducer and activator of transcription-1[J]. Diabetes, 2015, 64(12): 4285-4297.
[13] WANG E W, HAN Y Y, JIA X S. PAFR-deficiency alleviates myocardial ischemia/reperfusion injury in mice via suppressing inflammation, oxidative stress and apoptosis[J]. Biochem Biophys Res Commun, 2018, 495(4): 2475-2481.
[14] LIM W S, TIMMINS J M, SEIMON T A, et al. Signal transducer and activator of transcription-1 is critical for apoptosis in macrophages subjected to endoplasmic reticulum stress in vitro and in advanced atherosclerotic lesions in vivo[J]. Circulation, 2008, 117(7): 940-951.