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| Protective effect of paeonol on vascular endothelial cells damaged by hydrogen peroxide |
| PAN Da1, HU Feihong2, JIN Can1, WEN Hao1, MENG Weiyang1, ZHU Lielie1, CHEN Daqing1 |
| 1.Department of Emergency Medicine, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.Department of Spine Surgery, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China. |
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| Cite this article: |
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PAN Da,HU Feihong,JIN Can, et al. Protective effect of paeonol on vascular endothelial cells damaged by hydrogen peroxide[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(11): 797-801,806.
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Abstract Objective: To investigate the protective effects and mechanism of paeonol (PAE) on vascular endothelial cells injured by tert-butyl hydroperoxide (TBHP). Methods: Human umbilical vein endothelial cells (HUVECs) were randomly divided into control group (only treated with 1640 medium), injured group (stimulated by TBHP), and PAE treatment group (pretreated with various dose of PAE followed by TBHP stimulation).CCK-8 method and lactate dehydrogenase (LDH) release assay were used to detect the proliferation of HUVECs in each group. Transwell assay was used to detect the migration ability of HUVECs. Flow cytometry and immunofluorescence staining of Cleaved-caspase 3 were used to detect the apoptosis rate of HUVECs in each group. Results: Compared with the control group, the cell survival rate and cell migration number of the model group were significantly decreased, and the apoptosis rate, LDH release rate and the expression of Cleaved-caspase 3 protein were significantly increased (P<0.05). Compared with injured group, the survival rate and cell migration number of the PAE treatment group gradually increased in a dose-dependent manner; moreover, the apoptosis rate, LDH release rate and the expression of Cleaved-caspase 3 protein decreased in a dose-dependent manner (P<0.05). Conclusion: Paeonol can inhibit the TBHP-induced HUVECs viability due to the increase in LDH release and apoptosis, up-regulate the expression of Cleaved-caspase 3, and exert its protective effect on vascular endothelial cells injured by TBHP in a dose-dependent manner.
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Received: 22 April 2019
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