Alda-1通过激活线粒体乙醛脱氢酶2改善大鼠肺缺血再灌注损伤

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Abstract Objective: To investigate the protective effect of Alda-1 activated mitochondrial acetaldehyde dehydrogenase 2 (ALDH2) on lung ischemia-reperfusion injury (LIRI) in rats. Methods: Twenty-four SD male rats were randomly divided as sham operation group (Sham group), ischemia-reperfusion group (I/R group) and Alda-1+ischemia-reperfusion group (Alda-1 group). The lung tissue and arterial blood samples were taken at the end of the experiment. The wet-to-dry weight ratio (W/D) of the lung tissue was measured; the alveolar structure was observed by HE staining; the malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) in plasma and lung tissue were detected; the content of ALDH2 was determined by Western blot and the activity of ALDH2 by ELISA. Results: Compared with the Sham group, the I/R group showed that the W/D value was significantly increased (P<0.05), the alveolar structure markedly destroyed, MDA and 4-HNE in the plasma and lung tissues remarkably improved (P<0.05), the activity of ALDH2 signally decreased (P<0.05). However, in the Alda-1 group, the W/D value was significantly decreased (P<0.05), the alveolar structure markedly improved, the MDA and 4-HNE of plasma and lung tissues remarkably decreased (P<0.05), and the activity of ALDH2 signally improved (P<0.05), compared with the I/R group. Conclusion: Alda-1 can accelerate the metabolism of aldehydes by activating ALDH2 to improve lung ischemia-reperfusion injury.

Key words： lung reperfusion injury Alda-1 acetaldehyde dehydrogenase 2 malondialdehyde 4-hydroxy-nonenal

Received: 16 February 2019

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	ZHANG Yujian
	LIN Tingting
	XIA Fangfang
	DONG Jiaojiao
	JIN Zhousheng
	LIU Le

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I10/744

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