莪术醇对人卵巢癌SKOV3细胞增殖、迁移、侵袭及凋亡的影响

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1290 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the effects of curcumol on proliferation, migration, invasion and apoptosis of human ovarian cancer SKOV3 cells. Methods: The CCK-8 method was used to detect the inhibition rate of SKOV3 cells at different concentrations (0, 5, 10, 25, 50, 100 μmol/L) for 24 h and 48 h, and the IC50 value was calculated. The scratch test and Transwell chamber method were used to detect the cells. Migration and invasion ability, apoptosis rate was detected by flow cytometry, Western blot was used to detect the effects of curcumol and PI3K/AKT pathway blocker LY294002 on the expression of PI3K, p-PI3K, AKT and p-AKT proteins. Results: Curcumol significantly inhibited the proliferation of SKOV3 cells in a concentration-dependent but time-independent manner, with an IC50 value of 48 μmol/L. Meanwhile, curcumol significantly inhibited the migration and invasion of SKOV3 cells and promoted apoptosis (P<0.01). The expression of total protein PI3K and AKT was decreased (P<0.05), and the expression of phosphorylated forms p-PI3K and p-AKT was significantly decreased (P<0.01). The combination of curcumol and LY294002 showed a good synergistic effect, which was more obvious than that of the curcumol alone in the down-regulation of p-PI3K, p-AKT (P<0.05). Conclusion: Curcumol can inhibit the proliferation, migration and invasion of SKOV3 cells and induce apoptosis, which is related to the inhibition of PI3K/AKT pathway.

Key words： curcumol ovarian cancer proliferation migration invasion PI3K/AKT pathway

Received: 30 October 2018

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	DAI Linghong
	SUN Yun
	CHEN Xiangyan

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I10/740

[1] AI Z, LU Y, QIU S, et al. Overcoming cisplatin resistance of ovarian cancer cells by targeting HIF-1-regulated cancer metabolism[J]. Cancer Letters, 2016, 373(1): 36-44.
[2] LIN Y H, CHEN B Y, LAI W T, et al. The Akt inhibitor MK-2206 enhances the cytotoxicity of paclitaxel (Taxol) and cisplatin in ovarian cancer cells[J]. Naunyn Schmiedebergs Arch Pharmacol, 2015, 388(1): 19-31.
[3] KIGAWA J. New strategy for overcoming resistance to chemotherapy of ovarian cancer[J]. Yonago Acta Med, 2013, 56(2): 43-50.
[4] NING L, MA H, JIANG Z, et al. Curcumol suppresses breast cancer cell metastasis by inhibiting MMP-9 via JNK1/2 and Akt-dependent NF-κB signaling pathways[J]. Integr Cancer Ther, 2016, 15(2): 216-225.
[5] 潘睿. 中国慢性病前瞻性研究队列恶性肿瘤发病与死亡分析[D]. 南京: 南京医科大学, 2017.
[6] JANUCHOWSKI R, ZAWIERUCHA P, RUCIŃSKI M,
et al. Microarray-based detection and expression analysis of extracellular matrix proteins in drug-resistant ovarian cancer cell lines[J]. Oncol Rep, 2014, 32: 1981-1990.
[7] 秦铁城, 文海斌, 陈珮, 等. 莪术醇抗肿瘤研究进展[J]. 现代中西医结合杂志, 2013, 22(18): 2043-2045.
[8] 汤欣, 韩凤娟, 李威, 等. 莪术醇对人卵巢癌SKOV3细胞株JAK2/STAT3信号通路影响的研究[J]. 中国妇产科临床杂志, 2013, 14(1): 43-46.
[9] BOMMAREDDY A, CRISAMORE K, FILLMAN S, et al.
Survivin down-regulation by α-santalol is not mediated through PI3K-AKT pathway in human breast cancer cells[J].
Anticancer Res, 2015, 35(10): 5353-5357.
[10] 朱雪洁, 周璐璐, 朱雪琼. 晚期糖基化终末产物受体在妇科恶性肿瘤中的研究进展[J]. 温州医科大学学报, 2017, 47(10): 777-781.
[11] 韦立群, 李清, 甘嘉亮, 等. 迷迭香酸衍生物RAD-9通过PI3K/AKT和p38 MAPK信号通路诱导胃癌MGC-803细胞凋亡[J]. 中国药理学通报, 2018, 34(2): 256-260.
[12] YANG M, WANG H, ZHOU M. The natural compound sulforaphene, as a novel anticancer reagent, targeting PI3K-AKT signaling pathway in lung cancer[J]. Oncotarget, 2016, 7(47): 76656-76666.