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| Study on the effect and mechanism of ginsenoside Rg1 on survival and differentiation of implanted BMSCs in dementia model rats |
| 1.Department of brain center, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of neurology, Jinyun People’s Hospital, Lishui, 323000; 3.Department of hand surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027 |
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WU Wei1,CHEN Xiaohong2,YANG Jingquan3, et al. Study on the effect and mechanism of ginsenoside Rg1 on survival and differentiation of implanted BMSCs in dementia model rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(9): 637-.
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Abstract Objective: To explore the effect and mechanism of ginsenoside Rg1 on survival and differentiation of implanted BMSCs in dementia model rats. Methods: Forty five male SD rats were randomly divided into the FF transected model group (model group: ambi-hippocampal fimbria-fornix transected), the bone marrow mesenchymal stem cells (BMSCs treatment group): Two weeks after the model-made, every rat received transplantation of BMSCs (10 µL, 1×106 cells) into hippocampus on both sides by Hamilton microinjector with stereotaxis, combining group (ginsenoside Rg1 combining BMSCs treatment group) received both transplantation of BMSCs and peritoneal infusion of ginsenoside Rg1 (Two weeks after the model-made, every rat received peritoneal infusion of ginsenoside Rg1 (5 mg/kg), one day a time, until a month). Survival and migration of implanted BMSCs were observed by immunohistochemistry staining. Differentiation of BMSCs was observed using immunohistochemistry double staining. In order to explore the mechanism of ginsenoside Rg1 increased the number of implanted BMSCs, RT-PCR technique was used to detect the change of NGF mRNA expression in hippocampus. Results: Six weeks after the model-made, The number of BrdU positive cells in hippocampus of combining treatment group (34.2±5.4) was more than those of simple BMSCs treatment group (10.3±4.3), difference was significant (P<0.05). The level of NGFmRNA expression in hippocampus of combining treatment group (1.13±0.21) was higher than that of BMSCs treatment group (0.75±0.12), difference was significant. Conclusion: ginsenoside Rg1 is benefit to the survival of implanted BMSCs. Ginsenoside Rg1 may facilitate the survival of implanted BMSCs by up-regulating content of NGF mRNA in hippocampus
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Received: 25 January 2014
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[1]邬伟, 杨景全, 陈松芳, 等. 骨髓间充质干细胞移植对阿尔茨海默病大鼠学习记忆能力的影响[J]. 温州医学院学报,2008, 38(5): 412-414.
[2]邬伟, 闫峻, 张艳梅, 等. 人参皂苷Rg1诱导骨髓间充质干细胞分化为神经样细胞的作用[J]. 中风与神经疾病杂志,2007, 24(3): 282-284.
[3]Shichinohe H, Kuroda S, Sugiyama T, et al. Bone marrow stromal cell transplantation attenuates cognitive dysfunction due to chronic cerebral ischemia in rats[J]. Dement Geriatr Cogn Disord, 2010, 30(4): 293-301.
[4]Hellmann MA, Panet H, Barhum Y, et al. Increased survival and migration of engrafted mesenchymal bone marrow stem cells in 6-hydroxydopamine lesioned rodents[J]. Neurosci Lett, 2006, 395(2): 124-128.
[5]Liu Z, Li Y, Zhang ZG, et al. Bone marrow stromal cells en-hance inter- and intracortical axonal connections after isch-emic stroke in adult rats[J]. J Cereb Blood Flow Metab,2010, 30(7): 1288-1295.
[6]黄伟聪, 王珏, 杨凯, 等. rno-miRNA-133b对大鼠骨髓间充质干细胞增殖的影响[J]. 温州医学院学报, 2012, 42(5): 445-448.
[7]申丽红, 张均田. 人参皂苷Rgl对脑缺血沙土鼠神经干细胞存活率和学习记忆能力的影响[J]. 中南药学, 2003, 2(1): 6-9.
[8]Svendsen CN, Langston JW. Stem cells for Parkinson dis-ease and ALS: replacement or protection?[J]. Nat Med, 2004, 10(3): 224-225.
[9]Garcia R, Aguiar J, Alberti E, et al. Bone marrow stromal cells producenerve growth factor and glial cell line-derived neurotrophic factors[J]. Biochem Biophys Res Commun, 2004, 316(3): 753-754.
[10]Lu J, Moochhala S, Moore XL, et al. Adult bone marrow cells differentiate into neural phenotypes and improve func-tional recovery in rats following traumatic brain injury[J]. Neurosci Lett, 2006, 398(1-2): 12-17. |
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2014, Vol. 44 
