长链非编码RNA MIAT通过miR-124促进鼻咽癌细胞增殖、迁移和侵袭

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1444 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate whether long non-coding RNA MIAT regulates cell proliferation, migration and invasion in nasopharyngeal carcinoma cells through miR-124. Methods: qRT-qPCR was performed to detect MIAT expression in nasopharyngeal carcinoma cells. The interaction between MIAT and miR-124 was evaluated by dual luciferase reporter assay. si-MIAT and miR-124 inhibitor were transfected into nasopharyngeal carcinoma cells to detect their effects on proliferation, migration and invasion of nasopharyngeal carcinoma cells. The expression of Wnt/β-catenin signaling pathway was determined by Western blot. Results: Compared with normal nasopharyngeal epithelial cells NP-69, MIAT was significantly upregulated in nasopharyngeal carcinoma cell HONE-1. The online software and luciferase activity assay confirmed the interaction between MIAT and miR-124. Further experiments indicated that si-MIAT could increase the expression of miR-124, thus inhibiting cell proliferation, migration and invasion of nasopharyngeal carcinoma. The results of Western blot showed that MIAT functioned as an oncogene in nasopharyngeal carcinoma cell by up-regulating Wnt/β-catenin signaling pathway through miR-124. Conclusion: Our results demonstrated that MIAT affected proliferation, migration and invasion of nasopharyngeal carcinoma cells by regulating the expression of Wnt/β-catenin signaling pathway via miR-124. MIAT was a potential target for the diagnosis and treatment of nasopharyngeal carcinoma.

Key words： RNA, long non-coding MIAT miR-124 nasopharyngeal carcinoma

Received: 05 February 2019

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HAO Yalin
	JIN Xiaojie
	ZHAO Hui
	HE Ping
	ZHANG Jiafeng
	DONG Qiongna
	SHI Weiwei
	ZHAO Miaomiao

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I10/718

[1] CHUA M L, WEE J T, HUI E P, et al. Nasopharyngeal car-cinoma[J]. Lancet, 2016, 387(10022): 1012-1024.
[2] WEI W I, SHAM J S. Nasopharyngeal carcinoma[J]. Lancet, 2005, 365(9476): 2041-2054.
[3] HAN B L, XU X Y, ZHANG C Z, et al. Systematic review on Epstein-Barr virus (EBV) DNA in diagnosis of nasopharyngeal carcinoma in Asian populations[J]. Asian Pac J Cancer P, 2012, 13(6): 2577-2581.
[4] LEE N, XIA P, QUIVEY J M, et al. Intensity-modulated radiotherapy in the treatment of nasopharyngeal carcinoma: an update of the UCSF experience[J]. Int J Radiat Oncol Biol Phys, 2002, 53(1): 12-22.
[5] LI A C, XIAO W W, SHEN G Z, et al. Distant metastasis risk and patterns of nasopharyngeal carcinoma in the era of IMRT: long-term results and benefits of chemotherapy[J]. Oncotarget, 2015, 6(27): 24511-24521.
[6] LEE A W, NG W, HUNG W, et al. Major late toxicities after conformal radiotherapy for nasopharyngeal carcinoma-patient and treatment related risk factors[J]. Int J Radiat Oncol Biol Phys, 2009, 73(4): 1121-1128.
[7] XU S, WITMER P D, LUMAYAG S, et al. MicroRNA (miRNA) transcriptome of mouse retina and identification of a sensory organ-specific miRNA cluster[J]. J Biol Chem, 2007, 282(34): 25053-25066.
[8] JOUNG J, ENGREITZ J M, KONERMANN S, et al. Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood[J]. Nature, 2017, 548(7667): 343-346.
[9] YANG G, LU X, YUAN L. LncRNA: a link between RNA and cancer[J]. Biochim Biophys Acta, 2014, 1839(11): 1097-1109.
[10] SONG P, YIN S C. Long non-coding RNA EWSAT1 promotes human nasopharyngeal carcinoma cell growth in vitro by targeting miR-326/-330-5p[J]. Aging (Albany NY), 2016, 8(11): 2948-2960.
[11] LIU W, ZHANG Q, ZHANG J, et al. Long non-coding RNA MALAT1 contributes to cell apoptosis by sponging miR-124 in Parkinson disease[J]. Cell Biosci, 2017, 7: 19.
[12] WANG Y, LIN X, FU X, et al. Long non-coding RNA BANCR regulates cancer stem cell markers in papillary thyroid cancer via the RAF/MEK/ERK signaling pathway[J]. Oncol Rep, 2018, 40(2): 859-866.
[13] KONG Y G, CUI M, CHEN S M, et al. LncRNA-LINC00460 facilitates nasopharyngeal carcinoma tumorigenesis through sponging miR-149-5p to up-regulate IL6[J]. Gene, 2018, 639: 77-84.
[14] GUO J, MA J, ZHAO G, et al. Long noncoding RNA LINC0086 functions as a tumor suppressor in nasopharyngeal carcinoma by targeting miR-214[J]. Oncol Res, 2017, 25(7): 1189-1197.
[15] LIAO J, HE Q, LI M, et al. LncRNA MIAT: myocardial infarction associated and more[J]. Gene, 2016, 578(2): 158-161.
[16] CREA F, VENALAINEN E, CI X, et al. The role of epigenetics and long noncoding RNA MIAT in neuroendocrine prostate cancer[J]. Epigenomics, 2016, 8(5): 721-731.
[17] SHEN Y, DONG L F, ZHOU R M, et al. Role of long non-coding RNA MIAT in proliferation, apoptosis and migration of lens epithelial cells: a clinical and in vitro study[J]. J Cell Mol Med, 2016, 20(3): 537-548.
[18] ZHOU X, ZHANG W, JIN M, et al. lncRNA MIAT functions as a competing endogenous RNA to upregulate DAPK2 by sponging miR-22-3p in diabetic cardiomyopathy[J]. Cell Death Dis, 2017, 8(7): e2929.
[19] LI Y, WANG J, SUN L, et al. LncRNA myocardial infarction-associated transcript (MIAT) contributed to cardiac hypertrophy by regulating TLR4 via miR-93[J]. Eur J Pharmacol, 2018, 818: 508-517.
[20] ZHONG X, MA X, ZHANG L, et al. MIAT promotes proliferation and hinders apoptosis by modulating miR-181b/STAT3 axis in ox-LDL-induced atherosclerosis cell models [J]. Biomed Pharmacother, 2018, 97: 1078-1085.
[21] LI Y, WANG K, WEI Y, et al. lncRNA-MIAT regulates cell biological behaviors in gastric cancer through a mechanism involving the miR-29a-3p/HDAC4 axis[J]. Oncol Rep, 2017, 38(6): 3465-3472.
[22] LUAN T, ZHANG X, WANG S, et al. Long non-coding RNA MIAT promotes breast cancer progression and functions as ceRNA to regulate DUSP7 expression by sponging miR-155-5p[J]. Oncotarget, 2017, 8(44): 76153-76164.
[23] CALIN G A, CROCE C M. MicroRNA-cancer connection: the beginning of a new tale[J]. Cancer Res, 2006, 66(15): 7390-7394.
[24] DEL VESCOVO V, DENTI M A. microRNA and lung can-cer[J]. Adv Exp Med Biol, 2015, 889: 153-177.
[25] XIE B, DING Q, HAN H, et al. miRCancer: a microRNA-cancer association database constructed by text mining on literature[J]. Bioinformatics, 2013, 29(5): 638-644.
[26] LI S, MEI Z, HU H B, et al. The lncRNA MALAT1 contributes to non-small cell lung cancer development via modulating miR-124/STAT3 axis[J]. J Cell Physiol, 2018, 233(9): 6679-6688.
[27] FENG T, SHAO F, WU Q, et al. miR-124 downregulation leads to breast cancer progression via LncRNA-MALAT1 regulation and CDK4/E2F1 signal activation[J]. Oncotarget, 2016, 7(13): 16205-16216.
[28] CUI M, WANG J, LI Q, et al. Long non-coding RNA HOXA11-AS functions as a competing endogenous RNA to regulate ROCK1 expression by sponging miR-124-3p in os-teosarcoma[J]. Biomed Pharmacother, 2017, 92: 437-444.
[29] ZHANG T H, LIANG L Z, LIU X L, et al. Long non-coding RNA MALAT1 interacts with miR-124 and modulates tongue cancer growth by targeting JAG1[J]. Oncol Rep, 2017, 37(4): 2087-2094.
[30] HU H, WANG G, LI C. miR-124 suppresses proliferation and invasion of nasopharyngeal carcinoma cells through the Wnt/β-catenin signaling pathway by targeting Capn4[J].
Onco Targets Ther, 2017, 10: 2711-2720.