锌对邻苯二甲酸酯类暴露致雄性生殖毒性的干预作用

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Abstract Objective: To study the protective effect of zinc on reproductive toxicity caused by phthalate exposure in mice. Methods: Thirty-six male ICR mice were randomly divided into the control group, MIXPs (the equipotent toxic mixture of butyl benzyl phthalate (BBP), di (n-butyl) phthalate (DBP) and di (2-ethyhexyl) phthalate (DEHP) group, and MIXPs+ZnSO4 group, with 12 mice in each group. Mice in MIXPs group and MIXPs+ZnSO4 group were treated with 160 mg·kg-1·d-1 MIXPs by gavage with or without 50 mg·kg-1·d-1 ZnSO4. Mice in control group were treated with isodose 0.5% CMC-Na. After 90 days’ intervention, orbital vein blood was collected from each anesthetic mouse. Then dissect the mouse and take male reproductive organs such as the testicles and epididymis. Sperm morphology was observed with electron microscope. The levels of testosterone, SOD, MDA and T-AOC were measured with the commercial kits. Results: Compared with the control, the bodyweight, anogenital distance, serum testosterone, SOD and MDA in testicular tissue in MIXPs group were significantly reduced, while the rate of sperm deformation was significantly increased (P<0.05 or P<0.01), but there were no significant changes in MIXPs+ZnSO4 group. Conclusion: Zinc can inhibit the male reproductive toxicity induced by MIXPs exposure, which may be related to the improvement of the antioxidant level of testicular tissue and the increase of serum testosterone level.

Key words： zinc phthalates male reproductive toxicity antioxidant mice

Received: 28 November 2018

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	SUN Yue
	YANG Liu
	SHI Hongyi
	LI Anqi
	DAI Qimeng
	JIN Siyi
	GAO Haitao

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I9/649

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