TAZ在张应力促进人牙周膜细胞骨向分化中的作用

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Abstract Objective: To explore the role of PDZ binding transcriptional activator of transcription (TAZ) in tensile stress-induced cytoskeleton changes of human periodontal ligament cells (hPDLCs) to promote osteogenic differentiation. Methods: hPDLCs were obtained from PDL tissues in intact extracted third molars. Flexcell system was applied to construct the tensile stress loading model in vitro. Changes in TAZ and osteoblastic markers Cbfα1, collagen I (COL I), osteocalcin (OCN) and osterix (OSX) in hPDLCs were detected by Western blot and PCR. And the connection between TAZ and Cbfα1 was detected by co-immunoprecipitation. TAZ siRNA transfection was used to determine the role of TAZ in bone differentiation induced by tensile stress. Results: TAZ, Cbfα1, COL I, OSX and OCN mRNA and protein levels increased after 0.5 Hz and 12% tensile stress was applied to hPDLCs for 24 h and 48 h. There was a much more obvious connection between TAZ and Cbfα1 under tension for 24 h, compared with the control group. TAZ decreased after TAZ siRNA transfection in hPDLCs. Western blot results indicated that Cbfα1, COL I, OSX and OCN increased by tension were down-regulated after TAZ siRNA interference. Conclusion: TAZ induces osteogenic differentiation of human periodontal ligament cells by up-regulating Cbfα1 expression and transcriptional activity during tensile stress.

Key words： TAZ tensile stress core binding factor α1 human periodontal ligament cells osteogenic differentiation

Received: 29 April 2019

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	LU Suwen
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	DENG Hui

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I9/634

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