姜黄素类似物L6H3对帕金森病大鼠脑部纹状体中单胺类神经递质代谢物的影响

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Abstract Objective: To investigate the effect of curcumin analogue L6H3 on seven monoamine neurotransmitters and metabolite in the striatum of rats with Parkinson disease (PD) by micro-dialysis sampling coupled with high performance liquid chromatography and electrochemical detection (HPLC-ECD). Methods: PD model was established by injecting 6-OHDA into the right striatum of rats, then verified by the rotational behavior after intraperitoneal injection of apomorphine solution. Rats were randomly divided to Sham group, PD group and PD+L6H3 group. The microdialysis sampling coupled HPLC-ECD was developed and applied to detect the level of monoamine neurotransmitters and metabolite in rat striatum. Results: 6 weeks (2 weeks after administration),7 weeks (1 week after stopping administration) after model establishment, the levels of DA, DOPAC, HVA, NE, E, 5-HT, 5-HTAA in PD+L6H3 group were significantly increased compared with PD group (P<0.01). Conclusion: L6H3 has the capability to up-regulate monoamine neurotransmitters and metabolite in the striatum of PD rat model at both 2 weeks after administration and 1 week after stopping administration.

Key words： Parkinson disease striatum curcumin analogue high performance liquid chromatography and electrochemical detection monoamine neurotransmitter rats

Received: 24 December 2018

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	ZOU Yuchi
	CHEN Jun
	HUANG Wenting
	LIU Huan
	HU Jian
	LIU Fei
	WANG Dongxue
	ZHU Liyun
	LIN Li

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I9/625

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