复合软骨修复材料支架的构建

doi:10.3969/j.issn.2095-9400.2019.08.007

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Abstract Objective: To construct decellularized cartilage/GelMA composite scaffolds for repairing the cartilage defects. Methods: The α-1, 3-galactosidase gene knockout (GTKO) porcine costal cartilage was decellularized to obtain low immunogenic decellularized cartilage matrix. DAPI staining and DNA content detection were used to evaluate the effect of decellularization. HE staining and Masson trichrome staining were used to evaluate the extracellular matrix structure and protein retention. The decellularized cartilage was frozen and ground into powder, the polymer gelatin methacryloyl (GelMA) solution and decellularized cartilage powder were uniformly mixed in different proportion to prepare an injectable biomaterial, then obtained biodegradable composite scaffolds by ultraviolet crosslinking. Finally, the scaffolds were characterized by swelling performance, storage modulus and cell proliferation. Results: The DNA content of natural cartilage was 161.2 ng/mg, and the decellularized cartilage was only 15.27 ng/mg, so this method had a good effect on decellularization. HE staining showed that the extracellular matrix structure was intact, and Masson trichromatic staining showed that most of the extracellular matrix components were retained in decellularized cartilage. Compared with pure GelMA, the decellularized cartilage/GelMA composite scaffolds had lower swelling ratio, stronger mechanical strength. Bone marrow mesenchymal stem cells could adhere to scaffolds and proliferate. Conclusion: The scaffolds prepared by injectable decellularized cartilage/GelMA composite have low immunogenicity, suitable storage modulus, good biocompatibility, and are suitable for repairing cartilage defects.

Key words： extracellular matrix gelatin methacryloyl bone marrow mesenchymal stem cells cartilage repair low immunogenicity tissue engineering

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	WANG Yingying
	LI Huaqiong
	ZHAO Allan Zijian

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.08.007 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I8/581

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