miR-132通过降低HMGA2的表达抑制骨肉瘤细胞迁移、侵袭及上皮间质转化

doi:10.3969/j.issn.2095-9400.2019.08.005

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Abstract Objective: To investigate the effect of miR-132 on the migration, invasion and epithelial-mesenchymal transition (EMT) of osteosarcoma (OS) cells and explore the potential molecular mechanisms. Methods: The expression levels of miR-132 in human OS cells (U2OS and HOS) and human osteoblast hFOB1.19 were evaluated by quantitative real-time quantitative PCR (qRT-PCR). miR-132 mimic and miR-132 inhibitor were transfected into U2OS and HOS for the overexpression and knockdown of miR-132, respectively. qRT-PCR was employed to examine the efficacy of transfection. The effect of miR-132 on the migration, invasion and EMT of OS cells was detected by Transwell assay, qRT-PCR and Western blot. Bioinformatics website TargetScan was searched to identify the potential targets of miR-132 and dual luciferase reporter assay was performed to validate the interaction between miR-132 and HMGA2.Real-time quantitative PCR and Western blot were used to confirm the regulatory effect of miR-132 on HMGA2 expression. Results: The expression of miR-132 in OS cells was significantly decreased compared with that in hFOB1.19 cells (P<0.05). Transfection of miR-132 mimics significantly increased the expression level of miR-132 in U2OS cells (P<0.01). miR-132 overexpression led to decreased ability of cell migration and invasion, and increased E-cadherin level and decreased Vimentin level (P<0.05). Knockdown of miR-132 by transfection of miR-132 inhibitor resulted in enhanced metastatic ability, decreased E-cadherin expression and increased Vimentin expression (P<0.05). Data mining of bioinformatics website Targetscan suggested HMGA2 was a potential downstream target of miR-132. Dual luciferase reporter gene assay confirmed miR-132 could interact with HMGA2 3’-UTR. Overexpression of miR-132 significantly decreased HMGA2 expression while miR-132 knockdown led to increased expression of HMGA2 (P<0.05). Conclusion: The expression of miR-132 is decreased in OS cells, and miR-132 inhibits the migration, invasion and EMT of HCC cells by down-regulating the expression of HMGA2.

Key words： microRNA-132 osteosarcoma HMGA2 metastasis epithelial-mesenchymal transition

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	JIANG Zhen
	ZHANG Shuijun
	XU Jifeng
	ZHAO Xiaobo
	BAO Tao

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.08.005 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I8/568

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