大麻素II型受体参与调控低氧微环境下大鼠骨髓间充质干细胞的骨向分化

doi:10.3969/j.issn.2095-9400.2019.08.004

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Abstract Objective: To explore whether cannabinoid type 2 receptor (CB2) participates in the regulation of BMSCs osteogenic differentiation and how it is regulated by hypoxic microenvironment. Methods: The whole bone marrow cell adherence method was used to isolate and culture rBMSCs. Low-oxygen model was established by using different concentrations of chemical oxygen peroxide cobalt chloride (CoCl2). Real-time PCR and Western blotting were used to detect the expression of osteogenesis key proteins RUNX2 and OCN at the gene and protein levels. Further, combined with the CB2 inhibitor AM630, the role of CB2 in the osteogenic differentiation of rBMSCs in a hypoxic microenvironment was investigated. Results: Compared with the control group, the expression of RUNX2 and OCN mRNA and protein in rBMSCs increased significantly at 24, 48, 72, 96 h after hypoxia treatment (P<0.05); Oxygen treatment up-regulated mRNA and protein expression of CB2 (P<0.05). CB2 inhibitor AM630 inhibited the expression of RUNX2 and OCN induced by hypoxia (P<0.05). Conclusion: CB2 is involved in the regulation of osteogenic differentiation of rBMSCs promoted by hypoxia.

Key words： periodontitis rat bone mesenchymal stem cells cannabinoid type 2 receptor hypoxia osteogenic
differentiation

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	HU Xuzhi
	SHI Xinlian
	DENG Hui

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.08.004 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I8/563

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