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| The effect of Fasudil on degradation of chondrocyte matrix and its mechanism |
| JI Encheng1, Zhou Yeli1, Chen Chengwei1, Chen Chun1, Li Jing1, Gao Weiyang2, Pan Zhe’er1 |
| 1.Department of Orthopedics, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Department of Hand Surgery, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China |
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JI Encheng,Zhou Yeli,Chen Chengwei, et al. The effect of Fasudil on degradation of chondrocyte matrix and its mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(8): 557-562.
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Abstract Objective: To study the effect of Fasudil on matrix degradation of humolan chondrocytes and to explore its possible mechanism. Methods: CCK-8 was used to determine whether Fasudil was toxic and to determine the appropriate drug concentration. The cells were grouped as control group, Fasudil group, IL-1β group and Fasudil+IL-1β group. NO levels were determined by nitrate reductase assay; Western blot was used to detect COX-2, iNOS, MMP-13, p-MYPT, ROCK1, ROCK2, p-Erk/Erk, p-JNK/JNK and p-p38/p38 levels; qRT-MMP-13 and collagen-II expressions were determined by PCR; cell immunofluorescence was used to detect the expressions of MMP-13. Results: Compared with the IL-1β group, ROCK1, ROCK2 and p-MYPT were inhibited in the Fasudil+IL-1β group; the expression of COX-2, NO, iNOS and MMP-13 was inhibited, so did the expression of p-Erk, p-JNK and p-p38; the phosphorylation levels of Erk, JNK and p38 were decreased, and the expression of collagen-II was up-regulated. The differences were statistically significant (P<0.05). Conclusion: Fasudil inhibited IL-1β-induced degradation of chondrocyte matrix, which is probably achieved by inhibiting MAPK signaling pathway.
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