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| BC200 inhibits the temozolomide-induced apoptosis by mediating the maintenance of stemness of glioma cells |
| TONG Minfeng, YANG Fan, LIU Jihong, XU Hu |
| Department of Neurosurgery, Jinhua Municipal Central Hospital, Jinhua 321000, China |
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TONG Minfeng,YANG Fan,LIU Jihong, et al. BC200 inhibits the temozolomide-induced apoptosis by mediating the maintenance of stemness of glioma cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(7): 529-532,537.
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Abstract Objective: To investigate the role of serum long-chain non-coding RNA BC200 (lncRNA BC200) in glioma stem cells (GSCs) and related mechanism. Methods: Glioma U87 MG cells were cultured in neural stem cell culture medium and extracted into spherical growth GSCs. Immunofluorescence was used to detect the marker molecules CD133 and nestin of GSCs stem cells. Small RNA (siRNA) transfection silenced BC200 expression in GSCs. The GSCs were treated with Temozolomide (TMZ, 200 μmol·L-1) for 24 h, and the effect of TMZ on the morphology of suspended cells in GSCs was observed under light microscope. The expression of CD133, Bax, Bcl-2 and cleaved caspase-3 was detected by Western blot. Results: The extracted GSCs were isolated for spheroid growth, and the cell surface CD133 and nestin were positively expressed. After TMZ treatment of GSCs for 24 h, the ability of GSCs to form spheres did not change significantly, and the Bax, cleaved caspase-3 and Bcl-2 did not change significantly at 24 h. The expression of CD133 in GSCs was significantly decreased after silencing BC200. After BC200 silencing, TMZ treatment of GSCs showed that its ability to form a ball was significantly weakened, and the cells were dispersed. At 24 h, cleaved caspase-3 and Bax were up-regulated (45.36±4.25)% and (63.23±5.12)% (P<0.01). Bcl-2 was down-regulated (31.22±3.80)% (P<0.01). Conclusion: BC200 is able to maintain the dryness of glioma U87 MG cells, which promotes cell resistance to TMZ-induced apoptosis.
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Received: 12 November 2018
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