长链非编码RNA LINC00152在食管鳞癌组织中的表达及临床意义

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Abstract Objective: To explore the expression and clinical significance of long non-coding RNA (lncRNA) LINC00152 in esophageal squamous cell carcinoma. Methods: A total of 95 esophageal squamous cell carcinoma patients from the First Affiliated Hospital of Wenzhou Medical University during January 2008 to December 2013. LncRNA microarray gene chip technology was used to detect the expression level of lncRNA in the cancer tissue of esophageal squamous cell carcinoma and corresponding paracancerous normal tissues between metastatic group and non-metastatic group, in order to find the differential expression of lncRNAs. The differential expression of lncRNA in squamous cell carcinoma was verified by qRT-PCR. The correlation between lncRNA expression level and clinical features was analyzed. The ROC curve was drawn to evaluate the efficacy of LINC00152 as a predictor of esophageal squamous cell carcinoma metastasis. Correlation data of international gene chip lncRNA database of esophageal squamous cell carcinoma were retrieved to analyze the relationship between LINC00152 expression level and survival. Results: LncRNA microarray gene chip experiments showed that there were 10 lncRNAs in esophageal squamous cell carcinoma and paracancerous normal tissue with more than 5 times differential expression. The qRT-PCR validation of esophageal squamous cell carcinoma and paracancerous normal tissue found that the expression of 3 lncRNAs (MIR205HG, LINC00152, FOXD2-AS1) were statistically different between metastatic group and non-metastatic group. Statistical analysis revealed that the expression of LINC00152 was correlated with the lymph node metastasis of esophageal squamous cell carcinoma (P<0.01). ROC curve analysis showed that the AUC of ROC was 0.781(95%CI=0.512-0.824, P=0.032). International gene chip database data of esophageal cancer showed that the high expression of LINC00152 predicted a poor survival. Conclusion: LINC00152 may be involved in the metastasis process of esophageal squamous cell carcinoma and is a new biomarker for the prognosis of esophageal squamous cell carcinoma.

Key words： long non-coding RNA LINC00152 esophageal neoplasms metastasis

Received: 08 October 2018

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	YANG Yi
	LIN Xiaoming
	YIN Lihui
	YIN Qi
	LU Di
	SHAN Yunfeng

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I6/401

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