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| The effect of protectin DX on inflammation and fibroproliferation in primary lung fibroblasts |
| ZHENG Shengxing, PAN Jingyi, GU Lijun, YANG Jingxiang, CHEN Xinou, LI Ming, JIN Shengwei |
| Department of Anesthesiology and Perioperative Medicine, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China |
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| Cite this article: |
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ZHENG Shengxing,PAN Jingyi,GU Lijun, et al. The effect of protectin DX on inflammation and fibroproliferation in primary lung fibroblasts[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(6): 391-396.
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Abstract Objective: To explore effects of protectin DX (PDX) on the expression of cyclooxygenase-2 (COX-2), fibroproliferation, collagen production and myofibroblast differentiation in mice primary lung fibroblast cells (LF). Methods: After the lung fibroblasts were isolated and purified, the acute inflammation model and the fibriproliferation model was respectively established with lipopolysaccharide (LPS) and with TGF-β1. Then primary lung fibroblast cells were incubated in DMEM medium containing LPS or TGF-β1 in the presence or absence of PDX. After incubation, cell viability was measured using cell titer. mRNA expression was measured by realtime-PCR. The cells were harvested, and COX-2 protein was analyzed by western-blot. Supernatants from lung fibroblast cells were collected and PGE2 protein expression was measured by ELISA. Results: It is a reasonable model of fibroproliferation in fibroblasts by administering 10 ng/mL TGF-β1 for 48 hours. PDX reduced LPS-induced expression of COX-2 and production of PGE2, while inhibiting TGF-β1-induced lung fibroblast cells proliferation in a concentration dependent manner. In addition, PDX reduced rat primary LF collagen production, α-smooth muscle actin (α-SMA) and fibronectin expression induced by TGF-β1. Conclusion: PDX inhibits LPS-induced expression of COX-2 and production of PGE2 in primary lung fibroblast cells, while decreasing TGF-β1-induced fibroproliferation, collagen production and differentiation in primary lung fibroblast cells.
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Received: 03 February 2019
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